Rat anti Human CD28 antibody, clone YTH913.12 BIO-RAD MCA709PE Rat anti Human CD28 antibody, clone YTH913.12-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti Human CD28 antibody, clone YTH913.12 BIO-RAD MCA709PE Rat anti Human CD28 antibody, clone YTH913.12

2024-10-29

Rat anti Human CD28 antibody, clone YTH913.12

货号：MCA709PE

规格：100 Tests

市场价格：3893

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD28

物种：人

宿主：大鼠

抗体亚型：IgG2b

克隆号：YTH913.12

荧光染料：PE

产品介绍
Rat anti Human CD28 antibody, clone YTH913.12recognizes human CD28, a ~44 kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells (Thompsonet al.1989), responsible for activation of these cells via interaction with the TCR. CD28 is involved in the tuning of the T-cell for activation via TCR, lowering the threshold for activation from around 8000 triggered TCRs to approximately 1500 (Violaet al.1996).CD28 along with CD152, also known as CTLA-4 acts as a co-receptor for the co-stimulatory molecules CD80 and CD86 (Azumaet al.1993). CD28 offers a positive stimulatory role on ligation of CD80 and CD86 while CTLA-4 offers a negative feedback signal preventing CD28 mediated T-cell activation of CD86 (Krummelet al.1995).Rat anti human CD28, clone YTH913.12 has been reported to recognize an epitope of CD28 expressed by NK cells, which is not recognized by other anti human CD28 clones such as 9.3 and CD28.2 (Galea-Lauriet al1999.) Other reports however have failed to demonstrate CD28 staining on peripheral blood derived NK cells using clone YTH913.12 (
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1 ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Immunogen	Human peripheral blood T-cells
Fusion Partners	Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Reiter, C. (1989) Cluster Report: CD28 in Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr.Oxford University Press. pp 352-3.2.Galea-Lauri, J.et al.(1999) Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells.J Immunol. 163 (1): 62-70.3.Gabdoulkhakova, A.et al.(2007) High rate of mutation reporter gene inactivation during human T cell proliferation.Immunogenetics. 59: 135-43.4.Pridgeon, C.et al.(2011) Regulation of IL-17 in chronic inflammation in the human lung.Clin Sci (Lond). 120: 515-24.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Rat anti Human CD28 antibody, clone YTH913.12 BIO-RAD MCA709PE Rat anti Human CD28 antibody, clone YTH913.12

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