Mouse anti Pig CSF1R, clone ROS8G11 BIO-RAD MCA5957APC Mouse anti Pig CSF1R, clone ROS8G11-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Pig CSF1R, clone ROS8G11 BIO-RAD MCA5957APC Mouse anti Pig CSF1R, clone ROS8G11

2024-10-29

Mouse anti Pig CSF1R, clone ROS8G11

货号：MCA5957APC

规格：100 Tests

市场价格：4165

产品类型：流式抗体

品牌：Bio-Rad

抗原：CSF1R

物种：pig

宿主：小鼠

抗体亚型：IgG2a

克隆号：ROS8G11

荧光染料：APC

产品介绍
Mouse anti Pig CSF1R, clone ROS8G11, recognizes the porcine homologue of human CSF1R, also known as CSF-1R, M-CSF receptor, CD115 and c-fms. CSF1R (CD115) is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceauet al.2010).CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cell commitment and CSF1R expression appears primarily limited to macrophages (Garceauet al.2010). The development of the monoclonal antibody ROS8G11 specifically recognizing porcine CSF1R provides an antibody that will assist with research into porcine monocyte and macrophage biology (Moffatet al.2014). Clone ROS8G11 has been tested for cross reactivity in a limited number of species, and while it does not recognize human or equine CSFR1, it has been shown to recognize canine CD115.
产品详情
Target Species	Pig
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Reconstitution	Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose
Immunogen	Recombinant pCSF1R-Ig / Fc fusion protein
Fusion Partners	Spleen cells from immunized Balb/c mice were fused with cells of the Ba/F3 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
APC	650	661
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Moffatt, L.et al.(2014) Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115).Dev Comp Immunol. 47: 123-8.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Mouse anti Pig CSF1R, clone ROS8G11 BIO-RAD MCA5957APC Mouse anti Pig CSF1R, clone ROS8G11

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