Mouse anti Bovine CD21 monoclonal antibody, clone CC51 BIO-RAD MCA5953PE Mouse anti Bovine CD21 monoclonal antibody, clone C-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Bovine CD21 monoclonal antibody, clone CC51 BIO-RAD MCA5953PE Mouse anti Bovine CD21 monoclonal antibody, clone C

2024-10-29

Mouse anti Bovine CD21 monoclonal antibody, clone CC51

货号：MCA5953PE

规格：100 Tests

市场价格：4378

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD21

物种：Bovine

宿主：小鼠

抗体亚型：IgG2b

克隆号：CC51

荧光染料：PE

产品介绍
Mouse anti Bovine CD21 monoclonal antibody, clone CC51recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiplesushidomains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessenset al.1990). CD21 may be expressed on B-cells as either a long or a short form (Pringleet al.2012).Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkoraet. al.2013). In addition to clone CC51, clone CC21 (MCA1424GA) which has been demonstrated to recognise CD21 in a range of ruminant and other species is also available from Bio-Rad.
产品详情
Target Species	Bovine
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Immunogen	Porcine PBMCs
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Sinkora, M.et al.(2014) The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2.Vet Res. 45: 91.2.Sinkora, Met al.(2013) Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs.Dev Comp Immunol. 39: 409-18.3.Tenaya I.W.et al.(2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.Vet Immunol Immunopathol. 149: 167-76.4.Denham S.et al.(1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.Vet Immunol Immunopathol. 43: 259-67.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Mouse anti Bovine CD21 monoclonal antibody, clone CC51 BIO-RAD MCA5953PE Mouse anti Bovine CD21 monoclonal antibody, clone C

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