Mouse anti BRCA1, clone 17F8 BIO-RAD MCA5946GT Mouse anti BRCA1, clone 17F8-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti BRCA1, clone 17F8 BIO-RAD MCA5946GT Mouse anti BRCA1, clone 17F8

2024-10-29

Mouse anti BRCA1, clone 17F8

货号：MCA5946GT

规格：50 µg

市场价格：6800

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：BRCA1

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：17F8

荧光染料：Purified

产品介绍
Mouse anti BRCA1, clone 17F8recognizes both human and mouse BRCA1, otherwise known as breast cancer type 1 susceptibility protein, a tumor suppressor gene and major player in DNA damage repair, predominantly expressed in the nucleus during the S/G2 phase of the cell cycle.Along with BRCA2, BRCA1 is a high risk gene which is associated with hereditary breast and ovarian cancers, particularly at a younger age of diagnosis. Women carrying the BRCA1 mutation have a 50-95% chance of developing breast cancer in later life, but genetic screening and increased awareness of preventative surgery can reduce this risk significantly (Yamauchiet al.2018). Deleterious BRCA1 mutations may also increase the risk of other cancers in both males and females including pancreatic cancer, although in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations.BRCA1 is a key marker of triple-negative breast cancer/TNBC (ER-/PR-/HER2-), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and which lacks the benefit of specific therapy that targets the three major proteins ER/PR/HER2. Triple-negative tumors are predominantly basal-like, poorly differentiated and of higherhistological grade. Younger women have an increased rate of basal or BRCA related TNBC, compared with the higher proportion of apocrine, normal-like and rare subtypes of TNBC, seen in older women.Mouse anti BRCA1, clone17F8 recognizes full length 220 kDa BRCA1, but not the delta exon 11 splice variant.Mouse anti BRCA1, clone17F8 is well documented for use in immunohistochemistry and immunofluorescence staining, showning that this antibody labels both cytoplasmic BRCA1 and the centromeric region (overlapping with the nucleus), and exhibits cytoplasmic staining of BRCA1 in breast cancer cell lines and nuclear staining in several non-breast cancer cell lines (Lee,et al.1998).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)
Immunogen	E.coliexpressed BRCA1 protein fragment corresponding to amino acids 762-1315
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Lee, W.H.et al.(1998) Biological functions of the BRCA1 protein.Breast Dis. 10: 11-222.Maul, G.G.et al.(1998) Nuclear redistribution of BRCA1 during viral infection.Cell Growth Differ. 9 (9): 743-55.3.Zhong, Q.et al.(1999) Association of BRCA1 with the hRad50-hMre11-p95 complex and the DNA damage response.Science. 285 (5428): 747-50.

技术参数

产品应用 IF;WB

产品类型 Monoclonal Antibody

推荐用于 IF;WB

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Mouse anti BRCA1, clone 17F8 BIO-RAD MCA5946GT Mouse anti BRCA1, clone 17F8

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