Mouse anti Rat CD63 BIO-RAD MCA4754T Mouse anti Rat CD63

2024-10-29

Mouse anti Rat CD63

货号:MCA4754T,MCA4754GA

规格:25 µg,0.1 mg

市场价格:825,2023

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:CD63

物种:大鼠

宿主:小鼠

抗体亚型:IgG1, kappa

荧光染料:Alexa Fluor 488

产品介绍
Mouse anti Rat CD63 antibody, clone AD1recognizes rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, found on activated platelets and resident macrophages. Rat CD63 is expressed on mast cells in tissues and can be induced on other cell typesin vitro(Nishikataet al.1992).Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release and binding competition assays indicate that it is sterically situated close to the high-affinity IgE receptor on rat basophilic leukemia (RBL-2H3) cells (Kitaniet al.1991).
产品详情
Target SpeciesRat
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
ImmunogenRBL-2H3 rat basophilic leukaemia cell line.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8-653 myeloma cell line.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Kitani, S.et al.(1991) A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491.J Biol Chem. 266 (3): 1903-9.

技术参数

产品应用 Flow

产品类型 单克隆抗体

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