Mouse anti Rabbit GAPDH:DyLight®680 BIO-RAD MCA4739D680 Mouse anti Rabbit GAPDH:DyLight®680-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Rabbit GAPDH:DyLight®680 BIO-RAD MCA4739D680 Mouse anti Rabbit GAPDH:DyLight®680

2024-10-29

Mouse anti Rabbit GAPDH:DyLight®680

货号：MCA4739D680

规格：0.1 mg

市场价格：3213

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：GAPDH

物种：兔

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：Alexa Fluor 488

产品介绍
Mouse anti Rabbit GAPDH antibody, clone 6C5recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Haraet al.2006).
产品详情
Target Species	Rabbit
Product Form	Purified IgG conjugated to DyLight®680 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution	Phosphate buffered saline
Immunogen	Rabbit muscle GAPDH.
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Dylight®680	692	712
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Latasa, M.U.et al.(2010) Oral methylthioadenosine administration attenuates fibrosis and chronic liver disease progression in Mdr2-/- mice.PLoS One. 5: e15690.2.Haller, S.et al.(2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.Drug Metab Dispos. 40 (8): 1603-10.3.Zizza, P.et al.(2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.J Biol Chem. 287: 16849-59.4.Zschemisch, N.H.et al.(2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.BMC Immunol. 13: 60.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Mouse anti Rabbit GAPDH:DyLight®680 BIO-RAD MCA4739D680 Mouse anti Rabbit GAPDH:DyLight®680

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