mouse anti Rat MHC CLASS II RT1Bu/l:FITC 伯乐大鼠MHC CLASS II RT1Bu/l MCA45FT-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti Rat MHC CLASS II RT1Bu/l:FITC 伯乐大鼠MHC CLASS II RT1Bu/l MCA45FT

2024-10-29

mouse anti Rat MHC CLASS II RT1Bu/l:FITC

货号：MCA45FT

规格：0.1 mg

市场价格：2737

产品类型：流式抗体

品牌：Bio-Rad

抗原：MHC Class II RT1Bu/L

物种：大鼠

宿主：小鼠

抗体亚型：IgG1

克隆号：OX-3

荧光染料：FITC

产品介绍
Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3 also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.This product is routinely tested in flow cytometry on Lewis rat splenocytes.
产品详情
Target Species	Rat
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Immunogen	Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:FITC	MCA1209F	F	100 Tests/1ml
文献
1.McMaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.Eur J Immunol. 9 (6): 426-33.2.McMaster, W.R. & Williams, A.F. (1979) Monoclonal antibodies to Ia antigens from rat thymus: cross reactions with mouse and human and use in purification of rat Ia glycoproteins.Immunol Rev. 47: 117-37.3.Barclay, A.N. & Mayrhofer, G. (1981) Bone marrow origin of Ia-positive cells in the medulla rat thymus.J Exp Med. 153 (6): 1666-71.4.Zhang, J.et al.(1997) Expression of major histocompatibility complex molecules in rodent retina. Immunohistochemical study.Invest Ophthalmol Vis Sci. 38 (9): 1848-57.5.ahm, K.B.et al.(2000) Loss of TGF-beta signaling contributes to autoimmune pancreatitis.J Clin Invest. 105 (8): 1057-65.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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mouse anti Rat MHC CLASS II RT1Bu/l:FITC 伯乐 大鼠MHC CLASS II RT1Bu/l MCA45FT

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mouse anti Rat MHC CLASS II RT1Bu/l:FITC 伯乐大鼠MHC CLASS II RT1Bu/l MCA45FT