mouse anti Rat TCR alpha/beta:FITC 伯乐大鼠 TCR alpha/beta MCA453F-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti Rat TCR alpha/beta:FITC 伯乐大鼠 TCR alpha/beta MCA453F

2024-10-29

mouse anti Rat TCR alpha/beta:FITC

货号：MCA453F

规格：0.1 mg

市场价格：2661

产品类型：流式抗体

品牌：Bio-Rad

抗原： TCR alpha/beta

物种：大鼠

宿主：小鼠

抗体亚型：IgG1

克隆号：R73

荧光染料：FITC

产品介绍
Mouse anti Rat TCR alpha/beta antibody, clone R73recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colicet al.2010).
产品详情
Target Species	Rat
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Immunogen	Rat T Blasts and Erythrocytes.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
推荐产品
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:FITC	MCA1209F	F	100 Tests/1ml
文献
1.Ariki, S.G. and Roux, M.E. (1995) T lymphocyte subpopulations in the intestinal villi of immune deficient rats.In Recent Advances in Mucosal Immunology. Edited by McGhee, J., Mestecky, J., Tlaskalova, H. and Sterzl, J.2.Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.3.Hünig, T.et al.(1989) A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes.J Exp Med. 169 (1): 73-86.4.Tomida, S.et al.(1994) Intercellular adhesion molecule-1 and leukocyte function-associated antigen-1 are involved in protection mediated by CD3+TCR alpha beta- T cells at the early stage after infection with Listeria monocytogenes in rats.Int Immunol. 6 (7): 955-61.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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mouse anti Rat TCR alpha/beta:FITC 伯乐 大鼠 TCR alpha/beta MCA453F

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mouse anti Rat TCR alpha/beta:FITC 伯乐大鼠 TCR alpha/beta MCA453F