Mouse anti Rat CD86 伯乐Mouse anti Rat CD86 MCA2874-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Rat CD86 伯乐Mouse anti Rat CD86 MCA2874

2024-10-29

Mouse anti Rat CD86

货号：MCA2874,MCA2874GA,MCA2874T

规格：0.25 mg,0.1 mg,25 µg

市场价格：3018,2015,808

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD86

物种：大鼠

宿主：小鼠

抗体亚型：IgG1

克隆号：24F

荧光染料：Purified

产品介绍
Mouse anti Rat CD86 antibody, clone 24Frecognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages.Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production.Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86 (Maedaet al.1997).
产品详情
Target Species	Rat
Product Form	Purified IgG - liquid
Preparation	MCA2874GA, MCA2874: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant MCA2874T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Immunogen	HTLV-1 transformed Lewis-S1 cells.
Fusion Partners	Spleen cells from immunised Balb/c mice were fused with cells of the P3U1 mouse myeloma cell line.
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/25
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Maeda, K.et al.(1997) Characterization of rat CD80 and CD86 by molecular cloning and mAb.Int Immunol. 9 (7): 993-1000.2.Damoiseaux, J.G.et al.(1998) Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells.J Leukoc Biol. 64 (6): 803-9.3.Hanabuchi, S.et al.(2000) Development of human T-cell leukemia virus type 1-transformed tumors in rats following suppression of T-cell immunity by CD80 and CD86 blockade.J Virol. 74: 428-35.4.Kano, M.et al.(1998) A crucial role of host CD80 and CD86 in rat cardiac xenograft rejection in mice.Transplantation. 65: 837-43.5.Tamatani, T.et al.(2000) AILIM/ICOS: a novel lymphocyte adhesion molecule.Int Immunol. 12: 51-5.

技术参数

产品应用 IHC(F);Flow;IP

产品类型单克隆抗体

推荐用于 IHC(F);Flow;IP

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Mouse anti Rat CD86 伯乐Mouse anti Rat CD86 MCA2874

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