Mouse anti Ovine Interleukin-8 antibody, clone 8M6 伯乐 MCA1660 IL-8抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Ovine Interleukin-8 antibody, clone 8M6 伯乐 MCA1660 IL-8抗体

2024-10-29

Mouse anti Ovine Interleukin-8 antibody, clone 8M6

货号：MCA1660

规格：0.25 mg

市场价格：5670

产品类型：流式抗体

品牌：Bio-Rad

抗原： IL-8

物种：兔

宿主：小鼠

抗体亚型：IgG2a

克隆号：8M6

荧光染料：Purified

产品介绍
Mouse anti Ovine Interleukin-8 antibody, clone 8M6recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 79 amino acid ~9-11 kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti ovine Interleukin-8 antibody, clone 8M6 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.Responses to infectious stimuli may vary among ovine species, the response toMannheimia haemolytica, a causative agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exaggerated in Bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection (Herndonet al.2010).Mouse anti ovine interleukin-8 antibody, clone 8M6 has been utilized to identify cells and cell types expressing IL-8 in inflamed porcine tissue (Laursenet al.2014) showing here also that neutrophils are the predominant cell type expressing IL-8 whilst epithelial and endothelial cells in the vicinity of inflammatory lesions also express the cytokine. Clone 8M6 neutralizes the bioactivity of ovine IL-8.Mouse anti Ovine Interleukin-8 antibody, clone 8M6 has been used in conjunction with Rabbit anti Sheep Interleukin-8 antibody (AHP425) for the development of a sensitive ELISA to measure IL-8 concentrations in bovine samples (Croninet al.2015).
产品详情
Target Species	Sheep
Species Cross-Reactivity	Target Species	Cross Reactivity
Monkey	√
Dog	√
Rabbit	√
Bovine	√
Pig	√
Mustelid	√
Ferret	√
Mink	√
Cat	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)
Immunogen	Recombinant ovine IL-8.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹	√		1/10
ELISA	√		5ug/ml
Western Blotting	√
Functional Assays²	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm&trade: (Product CodeBUF09A) for this purpose.²Removal of the preservative is recommended prior to use in functional assays.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul. ELISAMouse anti Sheep Interleukin-8 antibody, clone 8M6 may be used in combination withAHP425in sandwich ELISA assays for ovine IL-8.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG2a Negative Control	MCA929	Flow	100 Tests
文献
1. Rainard, P.et al.(2008) The chemokine CXCL3 is responsible for the constitutive chemotactic activity of bovine milk for neutrophils.Mol Immunol. 45 (15): 4020-7.2. Stinson LFet al.(2014) Effects of cytokine-suppressive anti-inflammatory drugs on inflammatory activation inex vivohuman and ovine fetal membranes.Reproduction. 147 (3): 313-20.3. Tahoun, A.et al.(2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.Vet Res. 46: 9.4. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132 (2-4): 109-15.5. Erneholm, K.et al.(2016) Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection withChlamydia trachomatisserovar D.BMC Vet Res. 12 (1): 200.6. Melville, J.M.et al.(2017) Human amnion epithelial cells modulate the inflammatory response to ventilation in preterm lambs.PLoS One. 12 (3): e0173572.7. Magiri, R.et al.(2019) Innate immune response profiles in pigs injected with vaccine adjuvants polydi(sodium carboxylatoethylphenoxy)phosphazene (PCEP) and Emulsigen.Vet Immunol Immunopathol. 209: 7-16.

技术参数

产品应用 ELISA;FN;WB;Flow

产品类型 Monoclonal Antibody

推荐用于 ELISA;FN;WB;Flow

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Mouse anti Ovine Interleukin-8 antibody, clone 8M6 伯乐 MCA1660 IL-8抗体

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