mouse anti human CD3:StarBright Blue 700

货号：MCA463SBB700

规格：100 Tests/0.5ml

市场价格：3553

产品类型：流式抗体

品牌：Bio-Rad

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：其它

类型:	单抗	同型对照：	IgG1
浓度：		用法：	5 µL/test(Flow)
免疫原性：	Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.

Mouse anti Human CD3 antibody, clone UCHT1recognizes the human T-cell surface glycoprotein CD3 epsilon chain, also known as T-cell surface antigen T3/Leu-4 epsilon chain or CD3ε. CD3ε is a 207 amino acid, ~21kDa single pass type 1 transmembrane protein containing a singleIg-likeand a singleITAMdomain. Mouse anti Human CD3 antibody, clone UCHT1 was originally described as only binding to CD3ε when complexed with either the CD3δ or CD3γ subunits, as indicated by co-transfection immunofluorescence studies on COS cells (Salmerónet al.1991). Mouse anti Human CD3 antibody, clone UCHT1 binds to a region in the ectodomain of human CD3ε and has been shown to bind to adiscontinuous epitopenear an acidic region of CD3ε opposite the dimer interface; as shown by crystallographic studies of the CD3ε/δ dimer complexed with a single chain UCHT1 antibody fragment (Arnettet al.2004).CD3 is expressed by all T lymphocytes and is seen in all lymphoid organs including lymph nodes and spleen. It is involved in thymocyte differentiation (Brodeuret al.2009). Deficiency of the CD3ε chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency phenotype (Fischeret al.2005).Mouse anti Human CD3 antibody, clone UCHT1 has been used successfully for the activation of human peripheral blood lymphocytes by cross linking and subsequently for CD3ε surface espression by flow cytometry (Hirsh and Cohen 2006).

数据

Figure A. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and StarBright Violet 570 conjugated Mouse anti Human CD4 (MCA1267SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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参考文献：

1. Denning, S.M.et al. (1987) Activation of human thymocytes via CD3 and CD2 molecules.In McMichael, A.J.et al. Leucocyte Typing III: White Cell Differentiation Antigens. Edited by Oxford University Press pp 144-7.2. Harding, S.et al.(2002) A therapeutic CD4 monoclonal antibody inhibits TCR-zeta chain phosphorylation, zeta-associated protein of 70-kDa Tyr319 phosphorylation, and TCR internalization in primary human T cells.J Immunol. 169: 230-8.3. Hirsh, M.I.et al.(2006) Ultrastructural features of lymphocyte suppression induced by anthrax lethal toxin and treated with chloroquineLab Invest. 87: 182-8.4. Beverley, P.C. & Callard, R.E. (1981) Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.Eur J Immunol. 11 (4): 329-34.5. Kung, P.et al. (1979) Monoclonal antibodies defining distinctive human T cell surface antigens.Science. 206: 347-9.6. Clevers, H.et al.(1988) The transmembrane orientation of the epsilon chain of the TcR/CD3 complex.Eur J Immunol. 18 (5): 705-10.7. Salmerón, A.et al.(1991) A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.J Immunol. 147: 3047-52.8. Hirsh, M.I. and Cohen, V. (2006) Chloroquine prevents T lymphocyte suppression induced by anthrax lethal toxin.J Infect Dis. 194: 1003-7.9. Mahon, N.G.et al.(2002) Immunohistologic evidence of myocardial disease in apparently healthy relatives of patients with dilated cardiomyopathy.J Am Coll Cardiol. 39: 455-62.10. Campana, D.et al.(1987) The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage.J Immunol. 138 (2): 648-55.

技术参数

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