Mouse anti Rat CD25 antibody, clone OX-39 BIO-RAD MCA273R Mouse anti Rat CD25 antibody, clone OX-39

2024-10-29

Mouse anti Rat CD25 antibody, clone OX-39

货号:MCA273R,MCA273GA

规格:0.25 mg,0.1 mg

市场价格:3018,2015

产品类型:流式抗体

品牌:Bio-Rad

物种:大鼠

宿主:小鼠

抗体亚型:IgG1

克隆号:OX-39

荧光染料:Purified

产品介绍
Mouse anti Rat CD25 antibody, clone OX-39recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Patersonet al.1987).
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenStimulated Rat T cells
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the NS0/1 mouse myeloma cell line.
Preservative Stabilisers0.09% Sodium Azide (NaN3)
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/50
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Paterson, D.J.et al.(1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.Mol Immunol. 24 (12): 1281-90.2.Charteris DG & Lightman SL (1993)In vivolymphokine production in experimental autoimmune uveoretinitis.Immunology. 78 (3): 387-92.3.Hayosh, N.S. & Swanborg, R.H. (1987) Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors.J Immunol. 138 (11): 3771-5.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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