Rat anti mouse CD86:FITC 伯乐 Rat anti mouse CD86:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti mouse CD86:FITC 伯乐 Rat anti mouse CD86:FITC

2024-10-29

Rat anti mouse CD86:FITC

货号：MCA2463F

规格：0.1 mg

市场价格：2550

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD86

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b

克隆号：PO3

荧光染料：FITC

产品介绍
Rat anti mouse CD86 antibody, clone PO3recognizes mouse CD86 (B7-2), a ~80 kDa cell surface glycoprotein, a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses.Rat anti mouse CD86 antibody, clone PO3 has been reported to block some co-stimulatory functions of CD86 (Nakajima et al. 1997).
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners	Spleen cells from immunized Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
Buffer Solution	Phosphate buffered saline
Immunogen	Mouse B-cell line, BCL1.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Preservative Stabilisers	0.09%Sodium Azide1%Bovine Serum Albumin
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Jin, L.P.et al.(2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings.J Immunol. 173 (6): 3612-9.2.Nozawa, K.et al.(2001) Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases.J Immunol. 167 (9): 4981-6.3.Nakajima, A.et al.(1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells.Int Immunol. 9 (5): 637-44.4.Bedoret, D.et al.(2009) Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.J Clin Invest. 119 (12): 3723-38.5.Legutko, A.et al.(2011) Sirtuin 1 Promotes Th2 Responses and Airway Allergy by Repressing Peroxisome Proliferator-Activated Receptor-{gamma} Activity in Dendritic Cells.J Immunol. 187: 4517-29.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Rat anti mouse CD86:FITC 伯乐 Rat anti mouse CD86:FITC

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