Mouse anti human CD92 antibody, clone VIM-15b BIO-RAD MCA2521PE Mouse anti human CD92 antibody, clone VIM-15b

2024-10-29

Mouse anti human CD92 antibody, clone VIM-15b

货号:MCA2521PE

规格:100 Tests

市场价格:4038

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD92

物种:人

宿主:小鼠

抗体亚型:IgG2b

克隆号:VIM-15b

荧光染料:PE

产品介绍
Mouse anti human CD92 antibody, clone VIM-15brecognizes the C-terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70 kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells.CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine.Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10 (Wille, S.et al.). Removal of Sodium Azide is recommended prior to use in functional assays.
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
ReconstitutionReconstitute with 1.0ml distilled water
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose
Fusion PartnersSpleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at +4℃.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Wille, S.et al.(2001) Characterization of CDw92 as a member of the choline transporter-like protein family regulated specifically on dendritic cells.J Immunol. 167 (10): 5795-804.2.Majdic, O.et al. (1995) Leucocyte Typing V.Oxford University Press, Oxford pp.984-5.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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