mouse anti human CD146:Alexa Fluor® 647 伯乐MCA2141A647T 146抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti human CD146:Alexa Fluor® 647 伯乐MCA2141A647T 146抗体

2024-10-29

mouse anti human CD146:Alexa Fluor® 647

货号：MCA2141A647T ,MCA2141A647

规格：25 Tests/0.25ml,100 Tests/1ml

市场价格：1386,3188

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD146

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：OJ79c

荧光染料：Alexa Fluor 647

产品介绍
Mouse anti Human CD146 antibody, clone OJ79crecognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule (MCAM) or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single pass type 1 transmembrane glycoprotein with a calculated molecular mass of ~72 kDa. However due to extensive N-linked glycosylation CD146 migrates in polyacrylamide gels with an apparent molecular mass of ~118 kDa. CD146 is a member of the immunoglobulin superfamily bearing 2V-type Ig-likeand 3C-type Ig-like domains. CD146 is expressed by all endothelial cells and by melanoma cells and appears to act as an adhesion molecule (UniProt: P43121). Expression in melanoma may be linked to tumor progression (Lehmannet al.1989).Mouse anti Human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry (Chenet al.2014).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Pig	√
Dog	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Immunogen	Recombinant human MUC18 (D1-D5) Fc protein.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®647	650	665
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	F	100 Tests/1ml
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Human Seroblock	BUF070A	F	50 Test
Human Seroblock	BUF070B	F	200 Test
文献
1. James, A.W.et al.(2017) Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering.PLoS One. 12 (5): e0177308.2. Shen, J.et al.(2018) Effects of WNT3A and WNT16 on the Osteogenic and Adipogenic Differentiation of Perivascular Stem/Stromal Cells.Tissue Eng Part A. 24 (1-2): 68-80.3. Lapin, M.et al.(2017) Single-cell mRNA profiling reveals transcriptional heterogeneity among pancreatic circulating tumour cells.BMC Cancer. 17 (1): 390.4. Esteves, C.L.et al.(2017) Isolation and characterization of equine native MSC populations.Stem Cell Res Ther. 8 (1): 80.5. Gaceb, A.et al.(2017) Pericytes secrete pro-regenerative molecules in response to platelet-derived growth factor-BB.J Cereb Blood Flow Metab. : 271678X17719645.6. Noda, S.et al.(2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.Sci Rep. 9 (1): 5430.7. Gaceb, A.et al.(2020) AnIn Vitro.Partial Lesion Model of Differentiated Human Mesencephalic Neurons: Effect of Pericyte Secretome on Phenotypic Markers.J Mol Neurosci. 70 (11): 1914-25.8. Stefanska, A.et al.(2021) Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes.Methods Mol Biol. 2235: 169-180.9. Orikasa, S.et al.(2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.Sci Rep. 12 (1): 682.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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mouse anti human CD146:Alexa Fluor® 647 伯乐MCA2141A647T 146抗体

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