Mouse anti Pig CD16, clone G7 BIO-RAD MCA1971F Mouse anti Pig CD16, clone G7

2024-10-29

Mouse anti Pig CD16, clone G7

货号:MCA1971F

规格:0.1 mg

市场价格:3783

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD16

物种:pig

宿主:小鼠

抗体亚型:IgG1

克隆号:G7

荧光染料:FITC

产品介绍
Mouse anti Pig CD16, clone G7recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmulleret al.1998).Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierdaet al.1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloranet al.1994).
产品详情
Target SpeciesPig
Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide
ImmunogenPorcine peripheral blood leucocytes
Fusion PartnersSpleen cells from immunised Balb/c mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
Approx. Protein ConcentrationsIgG concentration 0.1mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Dato, M.E.et al.(1992) A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes.Cell Immunol. 140 (2): 468-77.2.Wierda, W.G.et al.(1993) Two distinct porcine natural killer lytic trigger molecules as PNK-E/G7 molecular complex.Cell Immunol. 146 (2): 270-83.3.Halloran, P.J.et al.(1994) Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7.Cell Immunol. 158 (2): 400-13.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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