| 产品介绍 | | |
| Mouse anti Human CD29 monoclonal antibody, clone 4B7Rrecognizes the human integrin beta 1 subunit, also known as CD29, a ~130 kDa (red) 115 kDa (non-red) single pass type I transmembrane glycoprotein expressed by most leucocytes andmesenchymal stem cells.Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Surface expression of CD29 on human natural killer cells can be reduced by pretreatment with the glutathione-S-transferase inhibitor diethyl maleate (Horvath-Arcidiaconoet al.2003) |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Alexa Fluor® 647 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin |
Immunogen | Occular melanoma cell line V+B2. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
Alexa Fluor®647 | 650 | 665 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 1 x 106cells in 100ul |
| 数据 |
| Figure A. FITC conjugated mouse anti human CD3 (MCA463F) and Alexa® 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti human CD3 (MCA463F) and Alexa® 647 conjugated mouse anti human CD19 (MCA1940A647). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A)Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse IgG1 isotype control (MCA928F) . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse anti Human CD29 (MCA1949F). All experiments performed on human blood gated on live, single mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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| Human Seroblock | BUF070A | F | 50 Test |
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| 文献 |
| 1. Tan, D.W.et al.(2013) Single-cell gene expression profiling reveals functional heterogeneity of undifferentiated human epidermal cells.Development. 140 (7): 1433-44.2. Yang, J.et al.(2015) Cordycepin protected against the TNF-α-induced inhibition of osteogenic differentiation of human adipose-derived mesenchymal stem cells.Int J Immunopathol Pharmacol. 28 (3): 296-307.3. Gu, Q.et al.(2015)Ginkgo bilobaextract promotes osteogenic differentiation of human bone marrow mesenchymal stem cells in a pathway involving Wnt/β-catenin signaling.Pharmacol Res. 97: 70-8.4. Chen, Y.et al.(2015) Effect of human umbilical cord mesenchymal stem cells transplantation on nerve fibers of a rat model of endometriosis.Int J Fertil Steril. 9 (1): 71-80.5. Lee, H.J.et al.(2017) ICOSL expression in human bone marrow-derived mesenchymal stem cells promotes induction of regulatory T cells.Sci Rep. 7: 44486.6. Yi, T.et al.(2015) Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.Tissue Eng Part C Methods. 21 (12): 1251-62.7. Sun, Y.et al.(2017) Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain.Pain Res Manag. 2017: 7346103.8. Kim, S.H.et al.(2019) Forkhead box O1 (FOXO1) controls the migratory response of Toll-like receptor (TLR3)-stimulated human mesenchymal stromal cells.J Biol Chem. 294 (21): 8424-37. |
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