Bio-Rad Actin Beta抗体 AHP2417,Rabbit anti Actin Beta

2024-10-22

Rabbit anti Actin Beta

货号:AHP2417

规格:0.1 mg

价格:2576

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Actin Beta

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍

Rabbit anti actin beta antibodyrecognizes the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic and beta actin1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modeling.Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant speciesArabidopsis thaliana(mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control.

产品详情

Target Species

Human

Species Cross-Reactivity

Target SpeciesCross Reactivity

Mouse √

Rat √

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography

AntiserumPreparation

Antiserum to actin beta was raised by repeated immunisation of rabbits with highly purified antigen

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1% Sodium Azide (NaN3)0.2% Gelatin

Immunogen

Peptide mapping to the terminal domain of human actin beta

Approx. Protein Concentrations

IgG concentration 0.2 mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

应用

Application Name

Verified

Min Dilution

Max Dilution

Western Blotting

1/200

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Western Blotting
  • Rabbit anti actin beta recognizes a band of appriximately 42 kDa when run under reducing conditions

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文献

1.Chen, X.J.et al.(2018) Association of reduced sclerostin expression with collapse process in patients with osteonecrosis of the femoral head.Int Orthop. 42 (7): 1675-82.2.Savova, M.S.et al.(2021) Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.Biomed Pharmacother. 141: 111934.

技术参数

产品应用 WB

产品类型 Polyclonal Antibody

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