mouse anti human CD49c:RPE 伯乐 MCA1948PE CD49c抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti human CD49c:RPE 伯乐 MCA1948PE CD49c抗体

2024-10-29

mouse anti human CD49c:RPE

货号：MCA1948PE

规格：100 Tests

市场价格：3936

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD49c

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：17C6

荧光染料：PE

产品介绍
Mouse anti Human CD49c antibody, clone 17C6recognizes CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c.Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line.
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1 ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
Immunogen	CD49c transfected cell line.
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Fusion Partners	Cells from immunised Balb/c mice were fused with cells of the NS2 mouse myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 monthsfrom dateof despatch
存储条件
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 5ul of the suggested working dilution to label 106cells in 100ul.
数据
Figure A. Alexa Fluor 647 conjugated Mouse anti Human EGFR (MCA1784A647) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B . Alexa Fluor 647 conjugated Mouse anti Human EGFR (MCA1784A647) and PE conjugated Mouse anti Human CD49c (MCA1948PE). All experiments performed on human peripheral blood gated on live single cells, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:PE	MCA928PE	Flow	100 Test
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文献
1. Nbaum, G.et al.(2006) Peptide p277 of HSP60 signals T cells: inhibition of inflammatory chemotaxis.Int Immunol. 18: 1413-9.2. Steinberg, F.et al.(2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.J Cell Biol. 197 (2): 219-30.3. Zanin-Zhorov, A.et al.(2003) T cells respond to heat shock protein 60 via TLR2: activation of adhesion and inhibition of chemokine receptors.FASEB J. 17: 1567-9.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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mouse anti human CD49c:RPE 伯乐 MCA1948PE CD49c抗体

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