mouse anti human CD147:APC 伯乐 MCA1876APC CD147抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti human CD147:APC 伯乐 MCA1876APC CD147抗体

2024-10-29

mouse anti human CD147:APC

货号：MCA1876APC

规格：100 Tests

市场价格：5908

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD147

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：MEM-M6/1

荧光染料：APC

产品介绍
Mouse anti Human CD147 antibody, clone MEM-M6/1recognizes the human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulatory factor. CD147 is a 385 amino acid single pass type 1 trans-membrane glycoprotein bearing 3 potential N-glycosylation sites, a single N-terminal (distal)Ig-like C2-type domainamd a more proximalIg-like V-type domainin its extracellular region. Mouse anti Human CD147 antibody, clone MEM-M6/1 was raised and screened against recombinant extracellular region of huCD147 expressed in CHO cells, along with a number of other MEM-M6 clones. Epitope mapping indicates that clone MEM-M6/1 binds to an epitope within the distal Ig-like C-2 type domain. This is confirmed by flow cytometry using beads coated with constructs containing only the distal Ig-like or proximal Ig-like domains (Kochet al.1999).Mouse anti Human CD147 antibody, clone MEM-M6/1 binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin (Kochet al.1999). In addition to clone MEM-M6/1 we are also able to offer theMEM-M6/6 clonederived from the same fusion which recognizes an epitope in the membrane proximal Ig-like V type domain and has inhibitory effects on CD3 induced T cell activation (Kock et al. 1999)
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
Reconstitution	Reconstitute with 1ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein A
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
Immunogen	CD147Rg, consisting of the cDNA coding for the extracellular region of human CD147.
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Fusion Partners	Spleen cells from immunized Balb/c mice were fused with SP2/0 mouse myeloma cells.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
APC	650	661
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
数据
Flow cytometric staining of human erythrocytes with Mouse anti Human CD147:APC (MCA1876APC).
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:APC	MCA928APC	Flow	100 Tests
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文献
1. Koch, C.et al.(1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density.Int Immunol. 11 (5): 777-86.2. Yang, Y.et al.(2008) Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis.Rheumatology (Oxford). 47: 1299-310.3. Schneiderhan, W.et al.(2007) Pancreatic stellate cells are an important source of MMP-2 in human pancreatic cancer and accelerate tumor progression in a murine xenograft model and CAM assay.J Cell Sci. 120: 512-9.4. Chen, Y.et al.(2009) Upregulation of HAb18G/CD147 in activated human umbilical vein endothelial cells enhances the angiogenesis.Cancer Lett. 278: 113-21.5. Schneiderhan, W.et al.(2009) CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro modelsGut. 58: 1391-8.6. Wright, G.J.et al.(2014) Immunogenic compositions and expression systems.Patent Publication number. US20140093540 A17. Huang, Z.et al.(2013) Overexpression of CD147 contributes to the chemoresistance of head and neck squamous cell carcinoma cells.J Oral Pathol Med. 42: 541-6.8. Hu, J.et al.(2010) Involvement of HAb18G/CD147 in T cell activation and immunological synapse formation.J Cell Mol Med. 14 (8): 2132-43.9. Bernard, S.C.et al.(2014) PathogenicNeisseria meningitidisutilizes CD147 for vascular colonization.Nat Med. 20 (7): 725-31.10. Nassif, X.et al.(2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia.Patent Publication number US20150110806 A111. Trakarnsanga, K.et al.(2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.Nat Commun. 8: 14750.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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mouse anti human CD147:APC 伯乐 MCA1876APC CD147抗体

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