mouse anti human CD326:RPE 伯乐 MCA1870PE CD326抗体

2024-10-29

mouse anti human CD326:RPE

货号:MCA1870PE

规格:100 Tests

市场价格:2567

产品类型:流式抗体

品牌:Bio-Rad

抗原: CD326

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:VU-1D9

荧光染料:PE

产品介绍
Mouse anti Human CD326 antibody, clone VU-1D9recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, andde novoexpression being seen in dysplastic squamous epithelium.
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

ReconstitutionReconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution

Preparation

Purified IgG prepared by affinity chromatography on Protein A

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin

5% Sucrose

ImmunogenHG9 cell line (small cell lung carcinoma)
Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Fusion PartnersSpleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RPE 561nm laser546578

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100u
数据

Figure A. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor 647 conjugated Mouse anti Human CD326 (MCA1870A647). All experiments performed on HT-29 cells gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:RPEMCA928PEFlow100 Tests
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文献
References for CD326 antibody1. Koga, Y.et al.(2008) Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads.Gastroenterol Res Pract. 2008: 605273.2. Fillmore, C.M.et al.(2010) Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling.Proc Natl Acad Sci U S A. 107 (50): 21737-42.3. Murakata, A.et al.(2011) Gene expression signature of the gross morphology in hepatocellular carcinoma.Ann Surg. 253: 94-100.4. Keller, P.J.et al.(2012) Defining the cellular precursors to human breast cancer.Proc Natl Acad Sci U S A. 109 (8): 2772-7.5. Jeong, H.T.et al.(2012) MRI features of hepatocellular carcinoma expressing progenitor cell markers.Liver Int. 32: 430-40.6. Armstrong, A.J.et al.(2019) Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.PLoS One. 14 (5): e0216934.Further Reading7. Winter, M.J.et al.(2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.Am J Pathol. 163 (6): 2139-48.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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