mouse anti M13 BACTERIOPHAGE 伯乐 MCA1858R 抗体

2024-10-29

mouse anti M13 BACTERIOPHAGE

货号:MCA1858R

规格:0.25 mg

市场价格:6571

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:其它

宿主:小鼠

抗体亚型:IgG

克隆号:E1

荧光染料:Purified

产品介绍
Mouse anti M13 Bacteriophage antibody, clone E1recognizes the gp3 (G3P) phage coat protein of the M13 bacteriophage widely used in the preparation of 'phage-display' immunoglobulin libraries. G3P plays a role in the entry of the viral genome into the bacterial host..This antibody may be used to detect binding of ScFv antibodies developed in the M13 system.
产品详情

Target Species

Viral

Product Form

Purified IgG- liquid

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN3)

ImmunogenM13 Phage coat proteins.
Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Regulatory

For research purposes only

Guarantee

Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA
Flow Cytometry10ug/ml
Immunohistology - Frozen
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
数据

Colo320 cell line stained with a cell-line specific M13 phage antibody (filled profile), compared to an irrelevant phage antibody (open profile). Cells were incubated overnight with 1012 phage particles, then detected with 10 ug/ml anti-M13 antibody (MCA1858R) followed by rabbit anti-mouse IgG:FITC (STAR9B)
文献
1. Jaye, D.L.et al.(2003) Use of real-time polymerase chain reaction to identify cell- and tissue-type-selective peptides by phage display.Am J Pathol. 162:1419-29.

技术参数

产品应用 ELISA;Flow;IHC(F)

产品类型 Monoclonal Antibody

推荐用于 ELISA;Flow;IHC(F)

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