Rat anti mouse CD34:PE 伯乐 MCA1825PE CD34抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti mouse CD34:PE 伯乐 MCA1825PE CD34抗体

2024-10-29

Rat anti mouse CD34:PE

货号：MCA1825PE

规格：100 Tests

市场价格：3188

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD34

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：MEC14.7

荧光染料：PE

产品介绍
Rat anti Mouse CD34 antibody, clone MEC14.7recognizes the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1.0 ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide1% Bovine Serum Albumin 5% Sucrose
Immunogen	T-end.1, a pMT transformed endothelial cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/5
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
数据
Staining of WEHI cells with Rat anti Mouse CD34: RPE (MCA1825PE)
同型
Description	Product Code	Applications	Pack Size
Rat IgG2a Negative Control:RPE	MCA1212PE	F	100 Tests
相关产品
Description	Product Code	Applications	Pack Size
Human Seroblock	BUF070A	F	50 Test
Human Seroblock	BUF070B	F	200 Test
文献
1. Winding, B.et al.(2002) Synthetic matrix metalloproteinase inhibitors inhibit growth of established breast cancer osteolytic lesions and prolong survival in mice.Clin Cancer Res. 8 (6): 1932-9.2. Nguyen, L.et al.(2012) Spatial morphological and molecular differences within solid tumors may contribute to the failure of vascular disruptive agent treatments.BMC Cancer. 12: 522.3. Morison, N.B.et al.(2007) The long-term actions of etonogestrel and levonorgestrel on decidualized and non-decidualized endometrium in a mouse model mimic some effects of progestogen-only contraceptives in women.Reproduction. 133: 309-21.4. Chen, L.et al.(2010) Roles of tetrahydrobiopterin in promoting tumor angiogenesis.Am J Pathol. 177: 2671-80.5. Ager, E.I.et al.(2010) Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases.Cancer Cell Int. 10: 196. Chabot, S.et al.(2011) A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor.J Exp Med. 208: 973-86.7. Chen, J.et al.(2011) Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in cerebral ischemic damage.Am J Physiol Endocrinol Metab. 2011 Jul;301(1):E62-71.8. Chen, J.et al.(2012) Transfusion of CXCR4-primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice.PLoS One. 7 (11): e50105.9. Ouji, Y. & Yoshikawa, M. (2016) Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro.Methods Mol Biol. 1516: 279-88.10. Nguyen, L.et al.(2016) Vascular disruptive agent OXi4503 and anti-angiogenic agent Sunitinib combination treatment prolong survival of mice with CRC liver metastasis.BMC Cancer. 16 (1): 533.11. Vávrová, J.et al.(2012) Irradiated stem cells and ageing of the haematopoietic system.Radiat Environ Biophys. 51 (2): 205-13.12. DaCosta, P.L.N.et al.(2018) The kallikrein-Kinin system modulates the progression of colorectal liver metastases in a mouse model.BMC Cancer. 18 (1): 382.13. Danielyan, L.et al.. (2020) Cell motility and migration as determinants of stem cell efficacyEBioMedicine. 60:102989.14. Rackham, C.L.et al.(2013) Maintenance of islet morphology is beneficial for transplantation outcome in diabetic mice.PLoS One. 8 (2): e57844.15. Fruchon, S.et al.(2012) Involvement of the Syk-mTOR pathway in follicular lymphoma cell invasion and angiogenesis.Leukemia. 26 (4): 795-805.16. Zhu, C.et al.(2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.J Neuroinflammation. 17 (1): 85.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Rat anti mouse CD34:PE 伯乐 MCA1825PE CD34抗体

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