CD3 Monoclonal Antibody (7D6)

货号：MHCD0300,MHCD0300-4

规格：0.5ml,2ml

价格：2346,8095

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG2a

克隆号： 7D6

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1:20(Flow)

靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

数据

CD3 Antibody (MHCD0300) in FlowFlow cytometry analysis of CD3 was performed in Jurkat cells. Cells were fixed with 1% paraformaldehyde for 20 mins and blocked in PBS containing 2.5% BSA for 45 minutes at room temperature. Cells were labeled with CD3 Mouse Monoclonal Antibody (MHCD03004, red histogram) at a dilution of 1:20 or a matched isotype control (pink histogram) in PBS. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature. A representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

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参考文献：

1.PloS oneIn Situ complement activation and T-cell immunity in leprosy spectrum: An immunohistological study on leprosy lesional skin."Published figure using CD3d monoclonal antibody (Product # MHCD0300) in Immunofluorescence"2.The Journal of clinical investigationBlocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs."MHCD03004 was used in flow cytometry to study the impact of modulating IFN-I signaling during suppressive combined antiretroviral therapy"3The Journal of experimental medicineDual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations."MHCD03004 was used in flow cytometry to identify RLTPR in patients and determine the effects of these mutations on CD4 positive T cells"4.PLoS pathogensTemporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection."MHCD0300 was used in flow cytometry to study the cytolytic effector capacity of HIV-specific CD8+ T cells"5.The Journal of infectious diseasesZoster Vaccination Increases the Breadth of CD4+ T Cells Responsive to Varicella Zoster Virus."MHCD0300 was used in flow cytometry to measure the memory CD4(+) T-cell responses to each varicella zoster virus protein at baseline and after zoster vaccination."

技术参数

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