Rat anti Human EGF Receptor:FITCBIO-RAD MCA1784F Rat anti Human EGF Receptor:FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti Human EGF Receptor:FITCBIO-RAD MCA1784F Rat anti Human EGF Receptor:FITC

2024-10-29

Rat anti Human EGF Receptor:FITC

货号：MCA1784F

规格：0.1 mg

市场价格：2865

产品类型：流式抗体

品牌：Bio-Rad

抗原：EGF Receptor

物种：人

宿主：大鼠

抗体亚型：IgG1

克隆号：ICR10

荧光染料：FITC

产品介绍
Rat anti Human EGF Receptor antibody, clone ICR10recognizes the human epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas.High level expression of EGFR is often associated with advanced disease and poor prognosis.Rat anti Human EGF Receptor antibody, clone ICR10 binds to epitope B from EGFR (Lottazet al.2010andModjtahediet al.1993) and has an affinity of 6.7 x 10-9M.
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Immunogen	Extracellular domain of human EGF-receptor from head and neck carcinoma.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/20
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Modjtahedi, H. & Dean, C. (1994) The receptor for EGF and its ligands - expression, prognostic value and target for therapy in cancer (review).Int J Oncol. 4 (2): 277-96.2.Lottaz,C.et al.(2010) Transcriptional Profiles of CD133+ and CD133- Glioblastoma-Derived Cancer Stem Cell Lines Suggest Different Cells of OriginCancer Res. 70: 2030-40.3.Modjtahedi, H.et al.(1993) Antitumor activity of combinations of antibodies directed against different epitopes on the extracellular domain of the human EGF receptor.Cell Biophys. 22 (1-3): 129-46.4.Modjtahedi, H.et al.(2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.Br J Cancer. 106 (5): 883-8.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Rat anti Human EGF Receptor:FITCBIO-RAD MCA1784F Rat anti Human EGF Receptor:FITC

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