Mouse anti Dog CD18:Alexa Fluor®647 BIO-RAD MCA1780A647 Mouse anti Dog CD18:Alexa Fluor®647-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Dog CD18:Alexa Fluor®647 BIO-RAD MCA1780A647 Mouse anti Dog CD18:Alexa Fluor®647

2024-10-29

Mouse anti Dog CD18:Alexa Fluor®647

货号：MCA1780A647

规格：100 Tests/1ml

市场价格：4280

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD18

物种：dog

宿主：小鼠

抗体亚型：IgG1

克隆号：CA1.4E9

荧光染料：Alexa Fluor 647

产品介绍
Mouse anti Dog CD18 antibody, clone CA1.4E9recognizes the canine CD18 cell surface antigen, also known as the b2 integrin.The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans (Mooreet al.1990) CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of Mouse anti Canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species.Immunoprecipitation experiments using detergent lysates of iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95 kDa beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180 kDa (CD11a ), 165 kDa (CD11b) and 150 kDa (CD11c) (Mooreet al.1990). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp (Fryet al.2003).High CD18 expression is a common feature of lymphoma and hyperplasia in dogs (Caniattiet al.1996)
产品详情
Target Species	Dog
Product Form	Purified IgG conjugated to Alexa Fluor® 647 - liquid
Species Cross-Reactivity	Target Species	Cross Reactivity
Horse	√
Human	√
Preparation	Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®647	650	665
Acknowledgements	This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Moore, P.F.et al.(1990) Canine leukocyte integrins: characterization of a CD18 homologue.Tissue Antigens. 36 (5): 211-20.2.Danilenko, D.M.et al. (1992) Canine leukocyte cell adhesion molecules (leuCAMS): characterization of the CD11/CD18 family.Tissue Antigens 40: 13-21.3.Brodersen, R.et al.(1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.Vet Immunol Immunopathol. 64 (1): 1-13.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Mouse anti Dog CD18:Alexa Fluor®647 BIO-RAD MCA1780A647 Mouse anti Dog CD18:Alexa Fluor®647

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