Rat anti Pig CD144 BIO-RAD MCA1748GA Rat anti Pig CD144-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti Pig CD144 BIO-RAD MCA1748GA Rat anti Pig CD144

2024-10-29

Rat anti Pig CD144

货号：MCA1748GA

规格：0.1 mg

市场价格：2185

产品类型：免疫组化一抗

品牌：Bio-Rad

物种：pig

宿主：大鼠

抗体亚型：IgG2a

克隆号：123

荧光染料：Purified

产品介绍
Rat anti Pig CD144 antibody, clone 123recognizes a ~120-130 kDa a single pass type I membrane protein considered to be the porcine homologue of human CD144, also known as VE-Cadherin or Cadherin-5.VE-cadherin is expressed by endothelial cells and is important in cell-cell adhesion, tight junction integrity and vascular permeabiltiy (Tinsleyet al.1999). VE-Cadherin is highly susceptible to proteolytic degradation.Western blotting and immunofluorescence using Rat anti Porcine CD144 antibody, clone 123 demonstrates rearrangement and upregulation of VE-cadherin in porcine endothelial cells cultured as part of tissue engineered blood vessels under shear stress conditions rather than static conditions (Leeet al.2012).
产品详情
Target Species	Pig
Product Form	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation	Purified IgG - liquid
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Carrier Free	Yes
Immunogen	Porcine VE-cadherin/human IgG Fc fusion protein.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Nasu, K.et al.(1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.Transplantation. 68 (6): 861-7.2.Allen, J.et al.(2008) Characterization of porcine circulating progenitor cells: toward a functional endothelium.Tissue Eng Part A. 14 (1): 183-94.3.Hoffmann, J.et al.(2008) Immobilized DNA aptamers used as potent attractors for porcine endothelial precursor cells.J Biomed Mater Res A. 84 (3): 614-21.4.Tsai, S.H.et al.(2007) Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering.Biochem Biophys Res Commun. 357 (4): 984-90.5.Zhong, A.et al.(2018) The Roles of Matrix Stiffness and β-Catenin Signaling in Endothelial-to-Mesenchymal Transition of Aortic Valve Endothelial Cells.Cardiovasc Eng Technol. 9 (2): 158-67.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Rat anti Pig CD144 BIO-RAD MCA1748GA Rat anti Pig CD144

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