RevertAid™ H Minus First Strand cDNA Synthesis Kit, with DNase I/RevertAid™第一链cDNA合成试剂盒(含DNase I) ThermocDNA合成 K16315

2024-10-29

RevertAid™ H Minus First Strand cDNA Synthesis Kit, with DNase I/RevertAid™第一链cDNA合成试剂盒(含DNase I)

货号:K16315,K16325

规格:20 reactions,100 reactions

市场价格:0,1687

产品类型:核酸合成

品牌:Thermo Fisher

The Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit (with DNase I) is a complete system for efficient synthesis of first-strand cDNA from RNA templates. The kit includes recombinant endonuclease (DNase I) to remove contaminating genomic DNA from RNA preps.For reverse transcription the kit uses RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

优点:

- Integrated genomic DNA removal step- High yields of full-length first-strand cDNA up to 13 kb- Increased reaction temperatures in the range of 42 to 55°C- Supplied with the recombinant RiboLock RNase Inhibitor- Complete kit—oligo(dT)18 and random hexamer primers included with the kit

组成成分:

▪1 x 25 µL RevertAid H Minus Reverse Transcriptase (200 U/µL)▪1 x 25 µL RiboLock RNase Inhibitor (20 U/µL)▪1 x 150 µL 5X Reaction Buffer▪1 x 50 µL dNTP Mix (10 mM)▪1 x 25 µL Oligo(dT)18 Primer (100 µM)▪1 x 25 µL Random Hexamer Primer (100 µM)▪1 x 20 µL Control GAPDH RNA (0.05 µg/µL)▪1 x 20 µL Forward GAPDH Primer (10 µM)▪1 x 20 µL Reverse GAPDH Primer (10 µM)▪2 x 1.25 mL water, nuclease-free▪1 x 20 µL DNase I, RNase-free (1 U/µL)Store at -20°C.

相关产品:

▪RevertAid cDNA第一链合成试剂盒(含DNase I)▪RevertAid H Minus第一链cDNA合成试剂盒(含DNase I)▪RevertAid 预混液(含DNase I)

技术参数

产品应用 • First strand cDNA synthesis for RT-PCR and real-time RT-PCR • Construction of full length cDNA libraries • Antisense RNA synthesis

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