Mouse anti Sheep Interleukin-6 antibody, clone 4B6 伯乐 MCA1659 IL-6-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Sheep Interleukin-6 antibody, clone 4B6 伯乐 MCA1659 IL-6

2024-10-29

Mouse anti Sheep Interleukin-6 antibody, clone 4B6

货号：MCA1659

规格：0.25 mg

市场价格：5670

产品类型：流式抗体

品牌：Bio-Rad

抗原：IL-6

物种：绵羊

宿主：小鼠

抗体亚型：IgG1

克隆号：4B6

荧光染料：Purified

产品介绍
Mouse anti Sheep Interleukin-6 antibody, clone 4B6recognizes ovine interleukin-6 (IL-6) and has also been reported to recognise recombinant human IL-6 and bovine IL-6 transfected cells. Mouse anti Sheep Interleukin-6 antibody, clone 4B6 does not cross react with ovine IL-1 beta, IL-8, MCP-1 or TNF alpha.
产品详情
Target Species	Sheep
Species Cross-Reactivity	Target Species	Cross Reactivity
Monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)
Immunogen	Recombinant ovine IL-6.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry 1	√
ELISA	√		5ug/ml (as a coating antibody)
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul. ELISAMCA1659 may also be used in combination withAHP424in a sandwich ELISA assays for ovine IL-6.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 Tests
文献
1. Herry, C.L.et al.(2016) Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.PLoS One. 11 (4): e0153515.2. Ciliberti, M.G.et al.(2017) Peripheral blood mononuclear cell proliferation and cytokine production in sheep as affected by cortisol level and duration of stress.J Dairy Sci. 100 (1): 750-756.3. Westover, A.et al.(2017) Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.Front Physiol. 8: 871.4. Castel, A.et al.(2021) Recording and manipulation of vagus nerve electrical activity in chronically instrumented unanesthetized near term fetal sheep.J Neurosci Methods. 360: 109257.5. Ciliberti, M.G.et al.(2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation usingin vitro.sheep model.J Dairy Sci. Mar 17 [Epub ahead of print].6. Cao, M.et al. (2022) The vagus nerve regulates immunometabolic homeostasis in the ovine fetus near term: impact on terminal ileumasXiv 2203.149677. Damiano, S.et al.(2022) Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambsIt J anim Sci 21 (1): 934-42.

技术参数

产品应用 ELISA;WB;Flow

产品类型 Monoclonal Antibody

推荐用于 ELISA;WB;Flow

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Mouse anti Sheep Interleukin-6 antibody, clone 4B6 伯乐 MCA1659 IL-6

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