Mouse anti Rat CD71 antibody, clone OX-26 伯乐 MCA155R CD71 抗体

2024-10-29

Mouse anti Rat CD71 antibody, clone OX-26

货号:MCA155R,MCA155G,MCA155GA

规格:0.25 mg,1mg,0.1mg

市场价格:3043,5457,2023

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD71

物种:大鼠

宿主:小鼠

抗体亚型:IgG2a

克隆号:OX-26

荧光染料:Purified

产品介绍
Mouse anti Rat CD71 antibody, clone OX-26recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues.The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis (Crihtonet al.1992).An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009).Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy (Lipardiet al.2002). OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195 kDa under non-reducing conditions reflecting it's homodimeric structure.
产品详情

Target Species

Rat

Product Form

Purified IgG - liquid

Preparation
  • MCA155G: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

  • MCA155R, MCA155GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

Carrier FreeYes
ImmunogenPHA activated rat lymphocytes.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Fusion PartnersSpleen cells from immunised Balb/c mice were fused with cells from the NS1 mouse myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry 11/50Pack Size: 1 mg, 0.1 mg1/10Pack Size: 0.25 mg1/200Pack Size: 1 mg, 0.1 mg1/100Pack Size: 0.25 mg
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG2a Negative ControlMCA1210Flow100 tests
文献

1.Moos, T.et al.(2003) Delivery of transferrin and immunoglobulins to the ventricular system of the rat.Front Biosci. 8: a102-9.2. Fabriek, B.O.et al.(2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.Blood. 109: 5223-9.3. Rathnasamy, G. et al.et al.(2011) Iron and Iron Regulatory Proteins in Amoeboid Microglial Cells Are Linked to Oligodendrocyte Death in Hypoxic Neonatal Rat Periventricular White Matter through Production of Proinflammatory Cytokines and Reactive Oxygen/Nitrogen SpeciesJ. Neurosci 31: 17982-95.4. Loureiro, J.A.et al.(2016) Cellular uptake of PLGA nanoparticles targeted with anti-amyloid and anti-transferrin receptor antibodies for Alzheimer's disease treatmentColloids and Surfaces B: Biointerfaces Apr 20 [Epub ahead of print]5. Loureiro, J.A.et al.(2015) Dual ligand immunoliposomes for drug delivery to the brain.Colloids Surf B Biointerfaces. 134: 213-9.6. Picard, E.et al.(2015) Targeting iron-mediated retinal degeneration by local delivery of transferrin.Free Radic Biol Med. 89: 1105-21.7. Ashrafzadeh, M.S.et al.(2020)In vivoGlioblastoma Therapy Using Targeted Liposomal Cisplatin.Int J Nanomedicine. 15: 7035-49.

技术参数

产品应用 IHC;IF; WB;Flow

产品类型 Monoclonal Antibody

推荐用于 IHC;IF; WB;Flow

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