Rat anti Human TNF alpha antibody, clone MP9-20A4 伯乐 MCA1560 TNF alpha抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti Human TNF alpha antibody, clone MP9-20A4 伯乐 MCA1560 TNF alpha抗体

2024-10-29

Rat anti Human TNF alpha antibody, clone MP9-20A4

货号：MCA1560

规格：0.5 mg

市场价格：4335

产品类型：流式抗体

品牌：Bio-Rad

抗原：TNF alpha

物种：人

宿主：大鼠

抗体亚型：IgG1

克隆号：MP9-20A4

荧光染料：Purified

产品介绍
Rat anti Human TNF alpha antibody, clone MP9-20A4recognizes human tumor Necrosis Factor - alpha (TNF alpha), also known as TNFSF2. This ~17kDa cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells.Rat anti Human TNF alpha antibody, clone MP9-20A4 is reported to act as a blocking antibody (Abramset al.2001).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide
Carrier Free	Yes
Approx. Protein Concentrations	1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry 1	√		Neat
ELISA	√	5ug/ml	10ug/ml
Immunohistology - Frozen	√
Western Blotting	X
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. The use of Leucoperm (Product CodeBUF09) is recommended for this purpose. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Rat IgG1 Negative Control	MCA6004GA	Flow	0.1 mg
数据
Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and purified Rat anti Human TNF alpha (MCA1560) detected with PE conjugated Goat anti Rat (STAR73). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and purified Rat IgG1 (MCA6004) detected with PE conjugated Goat anti Rat (STAR73). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and purified Rat anti Human TNF alpha (MCA1560) detected with PE conjugated Goat anti Rat (STAR73). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer
文献
1. Butler, D.M.et al.(1997) DBA/1 mice expressing the human TNF-alpha transgene develop a severe, erosive arthritis: characterization of the cytokine cascade and cellular composition.J Immunol. 159 (6): 2867-76.2. Vujanovic, L.et al.(2010) Virally infected and matured human dendritic cells activate natural killer cells via cooperative activity of plasma membrane-bound TNF and IL-15.Blood. 116: 575-83.3. Abrams, J.S. (2001) Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies.Curr Protoc Immunol. Chapter 6: Unit 6.20.4. Evans HGet al.(2009)In vivoactivated monocytes from the site of inflammation in humans specifically promote Th17 responses.Proc Natl Acad Sci U S A. 106 (15): 6232-7.5. Kobawala TPet al.(2016) Significance of TNF-α and the Adhesion Molecules: L-Selectin and VCAM-1 in Papillary Thyroid Carcinoma.J Thyroid Res. 2016: 8143695.6. Mann, E.R.et al.(2016) Compartment-specific immunity in the human gut: properties and functions of dendritic cells in the colon versus the ileum.Gut. 65 (2): 256-70.7. Kumagai, M.et al.(2012) Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination.J Control Release. 160 (3): 542-51.8. Poussin Cet al.(2014) Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in anin vitroassay revealed by transcriptomics analysis.Toxicol In Vitro. 28 (5): 896-908.

技术参数

产品应用 IHC;ELISA;Flow

产品类型 Monoclonal Antibody

推荐用于 IHC;ELISA;Flow

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Rat anti Human TNF alpha antibody, clone MP9-20A4 伯乐 MCA1560 TNF alpha抗体

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