mouse anti human CD105:Alexa Fluor 647 伯乐 MCA1557A647 CD105抗体

2024-10-29

mouse anti human CD105:Alexa Fluor 647

货号:MCA1557A647,MCA1557A647T

规格:100 Tests/1ml,25 Tests/0.25ml

市场价格:3188,1386

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD105

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:SN6

荧光染料:Alexa Fluor 647

产品介绍
Mouse anti Human CD105 antibody, clone SN6recognizes human endoglin, also known as CD105. CD105 is a glycoprotein homodimer of ~95 kDa subunits expressed by endothelial cells, activated monocytes and some leukemia cells.
产品详情

Target Species

Human

Species Cross-ReactivityTarget SpeciesCross Reactivity
Horse
Cynomolgus monkey
Rhesus Monkey
PrimateExpected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG conjugated to Alexa Fluor® 647- liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin

ImmunogenPartially purified cell membrane antigens from fresh leukemia cells
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®647650665

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/10Pack Size: 100 Tests/1ml1/5Pack Size: 25 Tests/0.25ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647Flow100 Tests/1ml
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数据

Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD105 (MCA1557A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD105 (MCA1557A647) and StarBright Violet 670 conjugated Mouse anti Human CD31 (MCA1738SBV670). All experiments performed on HUVECs gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
文献

1.Pierelli, L.et al.(2000) Modulation of bcl-2 and p27 in human primitive proliferating hematopoietic progenitors by autocrine TGF-beta1 is a cell cycle-independent effect and influences their hematopoietic potential.Blood 95: 3001-9.2.May, J.E.et al.(2018) Chemotherapy-induced genotoxic damage to bone marrow cells: long-term implications.Mutagenesis. 33 (3): 241-251.3.Hauser, P.V.et al.(2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.Am J Pathol. 177: 2011-21.4.Jin, H.J.et al.(2010) GD2 expression is closely associated with neuronal differentiation of human umbilical cord blood-derived mesenchymal stem cells.Cell Mol Life Sci. 67 (11): 1845-58.5.Nagano, M.et al.(2007) Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood.Blood 110 (1): 151-60.6.Braun, J.et al.(2010) Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells.Am J Vet Res. 71 (10): 1228-36.7.Diaz-Romero, J.et al.(2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.J Cell Physiol. 214: 75-83.8.Agha-Hosseini, F.et al.(2010)In vitroisolation of stem cells derived from human dental pulp.Clin Transplant. 24: E23-8.9.Arufe, M.C.et al.(2010) Chondrogenic potential of subpopulations of cells expressing mesenchymal stem cell markers derived from human synovial membranes.J Cell Biochem. 111: 834-45.10.Balmayor, E.R.et al.(2011) Synthesis and functionalization of superparamagnetic poly-ε-caprolactone microparticles for the selective isolation of subpopulations of human adipose-derived stem cells.J R Soc Interface 8: 896-908.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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