mouse anti human CD10:StarBright Violet 610 伯乐MCA1556SBV610 CD10抗体

2024-10-29

mouse anti human CD10:StarBright Violet 610

货号:MCA1556SBV610

规格:100 Tests/0.5ml

市场价格:3927

产品类型:流式抗体

品牌:Bio-Rad

抗原: SN5c

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:CD10

荧光染料:StarBright Violet 610

产品介绍
Mouse anti Human CD10 antibody, clone SN5c recognizes human neprilysin, also known as CD10, atriopeptidase, enkephalinase, neutral endopeptidase 24.11, skin fibroblast elastase or common acute lymphocytic leukemia antigen (CALLA). CD10 is a 749 aminoacid, ~100 kDa single pass type II transmembrane glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes.Defects in the MME gene encoding CD10 can lead to the development of the peripheral nervous system disorder, Charcot-Marie-Tooth disease 2T (CMY2T), an axonal form of Marie-Charcot-Tooth disease characterized by either dominantly inherited primary peripheral demyelinating neuropathies, designated CMT1, or primary peripheral axonal neuropathies showing axonal degeneration in the absence of any obvious myelin alteration (CMT2).
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to StarBright Violet 6100 - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

1% Bovine Serum Albumin

0.1% Pluronic F68

0.1% PEG 3350

0.05% Tween 20

ImmunogenPartially purified cell membrane antigens from fresh leukemia cells
Fusion PartnersSpleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
StarBright Violet 610402607

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
Store at +4°C. DO NOT FREEZE.This product should be stored undiluted.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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数据

Figure A. PE conjugated Mouse anti Human CD11b (MCA551PE). Figure B. PE conjugated Mouse anti Human CD11b (MCA551PE) and StarBright Violet 610 conjugated Mouse anti Human CD10 (MCA1556SBV610). All experiments performed on red blood lysed human blood gated on live single cell granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
文献

1.Biddle, W.C.et al.(1989)In vitroandin vivocytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates.Leuk Res. 13 (8): 699-707.2. Riemann, D.et al.(2001) Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains.Biochem J. 354 (Pt 1): 47-55.3. Diaz-Romero, J.et al.(2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflectbona fidededifferentiation rather than amplification of progenitor cells.J Cell Physiol. 214: 75-83.4. Pilling, D.et al.(2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.PLoS One. 4 (10): e7475.5. Manini, I.et al.(2020) Heterogeneity Matters: Different Regions of Glioblastoma Are Characterized by Distinctive Tumor-Supporting Pathways.Cancers (Basel). 12 (10)Oct 13 [Epub ahead of print].6. Glynn, E. & Fromm, J.R. (2020) Immunophenotypic Characterization and Purification of Neoplastic Cells from Lymph Nodes Involved by T-Cell/Histiocyte-rich Large B-cell Lymphoma by Flow Cytometry and Flow Cytometric Cell Sorting.Cytometry B Clin Cytom. 98 (1): 88-98.7. Caponnetto, F.et al.(2020) Human Adipose-Derived Stem Cells in Madelung's Disease: Morphological and Functional Characterization.Cells. 10 (1): 44.8. Kirolos, S.A. and Gomer, R.H. (2022) The extracellular sialidase NEU3 induces neutrophil primingbioRxiv. 24 Feb [Epub ahead of print].

技术参数

产品应用 Flow Cytometry

产品类型 Monoclonal Antibody

推荐用于 Flow Cytometry

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