mouse anti human CD104:Preservative Free 伯乐 MCA1456XZ CD104 抗体

2024-10-29

mouse anti human CD104:Preservative Free

货号:MCA1456XZ

规格:1 mg

市场价格:6401

产品类型:流式抗体

品牌:Bio-Rad

抗原: CD104

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号: 450-9D

荧光染料:Preservative Free

产品介绍
Mouse anti Human CD104 antibody, clone 450-9Drecognizes the human beta4 integrin, also known as CD104. CD104 is a ~205 kDa glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines.Mouse anti Human CD104 antibody, clone 450-9D recognizes an extracellular epitope on the CD104 molecule.
产品详情

Target Species

Human

Species Cross-ReactivityTarget SpeciesCross Reactivity
MustelidExpected from Sequence
Ferret
Mink
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

BufferSolution

Normal saline

Preservative Stabilisers

None present

Carrier FreeYes
ImmunogenPurified alpha6 beta4 integrin from A431 cells.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
Store at -20oC only.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow

1/251/100
Immunofluorescence
Immunohistology - Frozen11/5001/1000
Immunohistology - Paraffinx
Immunoprecipitation
Western Blotting
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
  • Histology Positive Control TissueHuman Tonsil
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative ControlMCA928Flow100 Tests
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文献
1. Kennel, S.J.et al.(1990) Second generation monoclonal antibodies to the human integrin alpha 6 beta 4.Hybridoma. 9 (3): 243-55.2. Barbaro, V.et al.(2009) Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes.Mol Vis. 15: 2084-93.3. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132:109-15.4. Smith, K.et al.(2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.Immunology. 132: 256-65.5. Alam, H.et al.(2011) Loss of keratins 8 and 18 leads to alterations in α6β4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line.J Cell Sci. 124: 2096-106.6. Lai-cheong, J.E.et al.(2009) Loss-of-function FERMT1 mutations in kindler syndrome implicate a role for fermitin family homolog-1 in integrin activation.Am J Pathol. 175 (4): 1431-41.7. Groves, R.W.et al.(2010) A homozygous nonsense mutation within the dystonin gene coding for the coiled-coil domain of the epithelial isoform of BPAG1 underlies a new subtype of autosomal recessive epidermolysis bullosa simplex.J Invest Dermatol. 130 (6): 1551-7.8. Aasted, B. & Viuff, B. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.Vet Immunol Immunopathol. 119 (1-2): 27-37.

    技术参数

    产品应用 IHC;IF;Flow;WB;IP

    产品类型 Monoclonal Antibody

    推荐用于 IHC;IF;Flow;WB;IP

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