Mouse anti Human mast cell tryptase, clone AA1 伯乐 MCA1438 Mast Cell Tryptase抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Human mast cell tryptase, clone AA1 伯乐 MCA1438 Mast Cell Tryptase抗体

2024-10-29

Mouse anti Human mast cell tryptase, clone AA1

货号：MCA1438,MCA1438T

规格：0.2 mg,20 µg

市场价格：893,3162

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Mast Cell Tryptase

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：AA1

荧光染料：Purified

产品介绍
Mouse anti Human mast cell tryptase, clone AA1recognizes human mast cell tryptase, bothalpha and betaisoforms. Mouse anti Mast cell tryptase, clone AA1 is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology (Wallset al.1990).Tryptases are the products of a number ofgenesand form the major neutral protease present in mast cells secreted in response to infection and injury. Mast cell tryptase has an important role in the pathology of inflammatory diseases, especially asthma through bronchoconstriction (Zhang and Timmerman 1997).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Dog	√
Monkey	√
Cat	√
Rat	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
Western Blotting	√
Immunofluorescence	√
Immunohistology - Frozen	√
Immunohistology - Paraffin1	√		1/10000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Histology Positive Control Tissue Tonsil
文献
1. Liu, J.et al.(2009) Genetic deficiency and pharmacological stabilization of mast cells reduce diet-induced obesity and diabetes in mice.Nat Med. 15: 940-5.2.Asano-Kato, N.et al.(2005) Tryptase increases proliferative activity of human conjunctival fibroblasts through protease-activated receptor-2.Invest Ophthalmol Vis Sci. 46: 4622-6.3. Facoetti, A.et al.(2006) Histochemical study of cardiac mast cells degranulation and collagen deposition: interaction with the cathecolaminergic system in the rat.Eur J Histochem. 50: 133-40.4. Dichlberger, A.et al.(2011) Lipid body formation during maturation of human mast cells.J Lipid Res. 52: 2198-208.5. Kawarai, S.et al.(2010) Cultivation and characterization of canine skin-derived mast cells.J Vet Med Sci. 72 (2): 131-40.6. Kazama, I.et al.(2015) Mast cell involvement in the progression of peritoneal fibrosis in rats with chronic renal failure.Nephrology (Carlton). 20 (9): 609-16.7. Kato, Y.et al.(2016) Cutaneous mastocytosis with a mutation in the juxtamembrane domain of c-kit in a young laboratory beagle dog.J Toxicol Pathol. 29 (1): 49-52.8. Luo, J.et al.(2016) An indispensable role of CPT-1a to survive cancer cells during energy stress through rewiring cancer metabolism.Tumour Biol. Oct 13 [Epub ahead of print].9. Perbellini, O.et al.(2011) Primary role of multiparametric flow cytometry in the diagnostic work-up of indolent clonal mast cell disorders.Cytometry B Clin Cytom. 80 (6): 362-8.10. Baba, A.et al.(2017) Less contribution of mast cells to the progression of renal fibrosis in Rat kidneys with chronic renal failure.Nephrology (Carlton). 22 (2): 159-67.

技术参数

产品应用 IHC;ELISA;IF;WB

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Mouse anti Human mast cell tryptase, clone AA1 伯乐 MCA1438 Mast Cell Tryptase抗体

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