IgM Monoclonal Antibody (II/41), FITC,

货号：11-5790-81

规格：50 µg

市场价格：883

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC
浓度：	0.5 mg/mL	用法：	0.25 µg/test(Flow)；10 µg/mL(ICC/IF)；Assay-Dependent(IHC)

产品详细信息Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: The II/41 antibody has been reported for use inflow cytometric analysis, immunohistochemical staining, and immunocytochemistry.Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This II/41 antibody has been tested by immunocytochemistry of fixed cells using a mouse IgM primary antibody. This antibody can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (11-5790-81) in FlowStaining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.125 µg of Anti-Mouse IgM FITC (right). Total viable cells were used for analysis.

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参考文献

1. AutophagyB cell autophagy mediates TLR7-dependent autoimmunity and inflammation.2. The Journal of experimental medicineElucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.3. GutEctopic lymphoid tissue alters the chemokine gradient, increases lymphocyte retention and exacerbates murine ileitis.4. Cell reportsChronic Viral Infection Promotes Efficient Germinal Center B Cell Responses.5. The FEBS journalTET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.

技术参数

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