Rat anti mouse CD117:Alexa Fluor® 488 伯乐 MCA1365A488T CD117抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti mouse CD117:Alexa Fluor® 488 伯乐 MCA1365A488T CD117抗体

2024-10-29

Rat anti mouse CD117:Alexa Fluor® 488

货号：MCA1365A488T,MCA1365A488

规格：25 Tests/0.25ml,100 Tests/1ml

市场价格：1250,3400

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD117

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b

克隆号： 2B8

荧光染料：Alexa Fluor 488

Mouse Seroblock FcR

产品介绍
Rat anti Mouse CD117 antibody, clone 2B8recognizes mouse CD117, a ~150 kDa cell surface molecule otherwise known as the Kit, or c-kit, receptor.Rat anti Mouse CD117 antibody, clone 2B8 recognizes an epitope within the extracellular region of CD117. In mice,CD117 is predominantly expressed on multipotent haematopoietic stem cells from the adult bone marrow and foetal liver.
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G.
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin
Immunogen	IL-3 dependent mast cells.
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Fusion Partners	Spleen cells from immunised Wistar rats were fused with cells of the Fox-NY mouse myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®488	495	519
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
相关产品
Description	Product Code	Applications	Pack Size
Mouse Seroblock FcR	BUF041A	F	0.1 mg
Mouse Seroblock FcR	BUF041B	F	0.5 mg
文献
1. Fleming WHet al.(1993) Steel factor influences the distribution and activity of murine hematopoietic stem cellsin vivo.Proc Natl Acad Sci U S A. 90 (8): 3760-4.2. Rico-Vargas, S.A.et al.(1994) c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody.J Immunol. 152 (6): 2845-52.3. Manova, K.et al.(1992) c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons.J Neurosci. 12 (12): 4663-76.4. Maeda, H.et al.(1992) Requirement of c-kit for development of intestinal pacemaker system.Development. 116 (2): 369-75.5. Takagi, M.et al.(1992) Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-kit receptor.J Immunol. 148 (11): 3446-53.6. de Vries, P.et al.(1992) Thymus reconstitution by c-kit-expressing hematopoietic stem cells purified from adult mouse bone marrow.J Exp Med. 176 (6): 1503-9.

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Rat anti mouse CD117:Alexa Fluor® 488 伯乐 MCA1365A488T CD117抗体

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