mouse anti CAT CD8 alpha/beta:PE 伯乐 MCA1347PE CD8 alpha/beta抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti CAT CD8 alpha/beta:PE 伯乐 MCA1347PE CD8 alpha/beta抗体

2024-10-29

mouse anti CAT CD8 alpha/beta:PE

货号：MCA1347PE

规格：100 Tests

市场价格：4378

产品类型：流式抗体

品牌：Bio-Rad

抗原： CD8 alpha/beta

物种：Cat

宿主：小鼠

抗体亚型：IgG1

克隆号： vpg9

荧光染料：PE

产品介绍
Mouse anti Cat CD8 alpha/beta antibody, clone vpg9recognizes the feline homolog of human CD8. Clone vpg 9 specifically recognizes an epitope associated with the alpha/beta complex of the CD8 heterodimer (Shimojimaet al.1998).
产品详情
Target Species	Cat
Species Cross-Reactivity	Target Species	Cross Reactivity
Human	X
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1 ml distilled water
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Immunogen	Feline thymocytes.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c were fused with cells of the NS0 mouse myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
Immunohistology - Frozen	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶lymphocytes in 100ul
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:RPE	MCA1209PE	F	100 Tests
Mouse IgG1 Negative Control:RPE	MCA928PE	F	100 Tests
数据
Cat lymphocytes stained with Mouse anti Cat CD8:Alpha/Beta (MCA1347GA) followed by Goat anti Mouse IgG:FITC (STAR117F); dual stained with Mouse anti Cat CD4:RPE
文献
1. Pistello, M.et al.(2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.J Virol. 84: 3845-56.2. Willett, B.J.et al.(2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.J Virol. 81: 9665-79.3. Willett, B.J.et al.(2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.PLoS One. 8 (1): e54871.4. Milner, R.J.et al.(2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.J S Afr Vet Assoc. 75 (2): 103-7.5. Rydzewski, L.et al.(2016) Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability.Vet Immunol Immunopathol. 177: 24-34.6. de Oliveira Medeiros, S.et al.(2016) Follow-up on long-term antiretroviral therapy for cats infected with feline immunodeficiency virus.J Feline Med Surg. 18 (4): 264-72.7. Guzera, M.et al.(2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.Vet Comp Oncol. 14 Suppl 1: 40-51.8. O'Brien, T.et al.(2015) Moderate dietary supplementation with vitamin E enhances lymphocyte functionality in the adult cat.Res Vet Sci. 99: 63-9.9. Sparger, E.E.et al.(2018) Investigation of immune cell markers in feline oral squamous cell carcinoma.Vet Immunol Immunopathol. 202: 52-62.10.Maeta, N.et al.(2019) Lymphokine-activated killer cell transplantation after anti-cancer treatment in two aged cats.Open Vet J. 9 (2): 147-50.11. Mustaffa-Kamal, F.et al.(2019) Characterization of antiviral T cell responses during primary and secondary challenge of laboratory cats with feline infectious peritonitis virus (FIPV).BMC Vet Res. 15 (1): 165.

技术参数

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mouse anti CAT CD8 alpha/beta:PE 伯乐 MCA1347PE CD8 alpha/beta抗体

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