| 产品介绍 | | |
| Mouse anti Cat MHC Class II antibody, clone vpg3recognizes a monomorphic determinant on feline MHC class II molecules. MonomorphicThe major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In cats, this is referred to as the feline leukocyte antigen (FLA) region.Mouse anti Cat MHC Class II antibody, clone vpg3 recogniszes monomorphic feline MHC class II molecules which are expressed by antigen presenting cells, B cells, monocytes and both activated and resting T lymphocytes. |
| 产品详情 | | |
Target Species | Cat |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Human | X |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Tissue Culture Supernatant - liquid |
Preparation | Tissue Culture Supernatant containing 10mM HEPES and5-10% foetal calf serum |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | IL-2 dependent feline T cells. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the NS0 mouse myeloma cell line |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | Neat |
| Immunohistology - Frozen1 | √ | | |
| Immunohistology - Paraffin | X | | |
| Immunoprecipitation | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106lymphocytes in 100ul |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
| 文献 |
| 1. Willett, B.J.et al. (1993) Infection with feline immunodeficiency virus is followed by the rapid expansion of a CD8+ lymphocyte subset.Immunology 78: 1-6.2. Willett, B.J., and Callanan, J.J. (1995) The expression of leucocyte differentiation antigens in the feline immune system., p 3-15. In Feline Immunology and Immunodeficiency (eds.) Willett, B.J. and Jarrett, O.Oxford University Press.3. Beatty, J.A.et al.(1996) A longitudinal study of feline immunodeficiency virus-specific cytotoxic T lymphocytes in experimentally infected cats, using antigen-specific induction.J Virol. 70 (9): 6199-206.4. Avery, P.R.et al.(2007) Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.Vet Immunol Immunopathol. 117 (3-4): 222-35.5. Mumaw, J.L.et al.(2015) Feline mesenchymal stem cells and supernatant inhibit reactive oxygen species production in cultured feline neutrophils.Res Vet Sci. 103: 60-9.6. Hein, A.et al.(2003) Ramified feline microglia selects for distinct variants of feline immunodeficiency virus during early central nervous system infection.J Neurovirol. 9 (4): 465-76. |
QQ:1749072012