mouse anti Rat CD31:RPE 伯乐 MCA1334PE CD31抗体

2024-10-29

mouse anti Rat CD31:RPE

货号:MCA1334PE

规格:100 Tests

市场价格:3596

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD31

物种:大鼠

宿主:小鼠

抗体亚型:IgG1

克隆号:TLD-3A12

荧光染料:PE

产品介绍
Mouse anti Rat CD31 antibody, clone TLD-3A12recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S.et al.2009).Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilsonet al.2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Macleanet al.2001)
产品详情

Target Species

Rat

Species Cross-ReactivityTarget SpeciesCross Reactivity
Rhesus Monkey
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
ReconstitutionReconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

1% Bovine Serum Albumin

5% Sucrose

ImmunogenActivated, Lewis rat derived microglial cells.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578

Fusion Partners

Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry

Use 10ul of the suggested working dilution to label 106cellsin 100ul.
同型
DescriptionProduct CodeApplicationsPack Size

Mouse IgG1 Negative Control:RPE

MCA1209PEF100 Tests/1ml
文献
1. Aminzadeh, A.et al.(2020) Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.Cell J. 22 (Suppl 1): 38-48.2. Pedram, M.S.et al.(2010) Transplantation of a combination of autologous neural differentiated and undifferentiated mesenchymal stem cells into injured spinal cord of rats.Spinal Cord. 48 (6): 457-63.3. Sheu, J.J.et al.(2012) Combination of cilostazol and clopidogrel attenuates rat critical limb ischemia.J Transl Med. 10: 164.4.Costa, B.P.et al.(2018) Intestinal Epithelial Stem Cells: Distinct Behavior After Surgical Injury and Teduglutide Administration.J Invest Surg. 31 (3): 243-52.5. Pun, C.K.et al.(2021) Glycyrrhizin Attenuates Portal Hypertension and Collateral Shunting via Inhibition of Extrahepatic Angiogenesis in Cirrhotic Rats.Int J Mol Sci. 22(14):7662.6. Huang, H.C.et al.(2021) Matrix metalloproteinase-9 inhibition or deletion attenuates portal hypertension in rodents.J Cell Mol Med. 25 (21): 10073-87.7. Huang, H.C.et al.(2021) Microbiota transplants from feces or gut content attenuated portal hypertension and portosystemic collaterals in cirrhotic rats.Clin Sci (Lond). 135 (24): 2709-2728.8. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with SaprophyticStaphylococcus epidermidis..Biomedicines. 10 (5): 1021.

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