mouse anti human CD33:StarBright Violet 515

货号：MCA1271SBV515

规格：100 Tests/0.5ml

市场价格：3851

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD33

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：WM53

荧光染料：StarBright Violet 515

类型：	单抗	同型对照：	IgG1
浓度：		用法：	5.0 µl/test(Flow)
免疫原性：	Human AML cells

Mouse anti Human CD33 antibody, clone WM53recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin.Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets.

数据

Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A647). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A647) and SBV515 conjugated Mouse anti Human CD33 (MCA1271SBV515). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on theZE5 Cell Analyzer.

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参考文献：

1.Favaloro, E.J.et al.(1987) Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, gp67 (CD-33).Dis Markers. 5 (4): 215-25.2.Favaloro, E.J.et al.(1988) Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33.Br J Haematol. 69 (2): 163-71.3.Hernández-Caselles, T.et al.(2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.J Leukoc Biol. 79: 46-58.4.Biedermann, B.et al.(2006) Analysis of the CD33-related siglec family reveals that Siglec-9 is an endocytic receptor expressed on subsets of acute myeloid leukemia cells and absent from normal hematopoietic progenitors.Leuk Res. 31: 211-20.5.Lajaunias, F.et al.(2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling.Eur J Immunol. 35: 243-51.6.Pietschmann, P.et al.(2000) Surface markers and transendothelial migration of dendritic cells from elderly subjects.Exp Gerontol. 35: 213-24.7.Favaloro, E.J.et al. (1993) Differential expression of surface antigens on activated endothelium.Immunol Cell Biol. 71:571-81.8.Yasukawa, T.et al.(2012) Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis.Anal Chem. 84 (20): 8830-6.9.Hu, Z.et al.(2016) Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibodyJ Wuhan Univ of Technol-Mater. Sci. Ed. 31 (2): 446-53.10.Dahl Cet al.(2004) Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells culturedin vitro.Allergy. 59 (10): 1087-96.

技术参数

产品应用 FLOW

产品类型 Monoclonal Antibody

推荐用于 FLOW

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