Rat anti Mouse CD25:StarBright Violet 440

货号：MCA1260SBV440

规格：100 Tests/0.5ml

市场价格：5075

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD25

物种：小鼠

宿主：大鼠

抗体亚型：IgG1

克隆号：PC61.5.3

荧光染料：StarBright Violet 440

类型:	单抗	同型对照：	IgG1
浓度：		用法：	5.0 µl/test(Flow)
免疫原性：	B6.1 CTL cell line.

Rat anti Mouse CD25 antibody, clone PC61.5.3reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation.

数据

Figure A. StarBright Blue 700 conjugated Rat anti Mouse CD45RA (MCA1258SBB700). Figure B. StarBright Blue 700 conjugated Rat anti Mouse CD45RA (MCA1258SBB700) and StarBright Violet 440 conjugated Rat anti Mouse CD25 (MC1260SBV440). All experiments performed on Mouse splenocytes treated with Concanavalin A for 24 hrs cells gated on live single cells, in the presence of 10% mouse serum. Data acquired on theZE5 Cell Analyzer.

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参考文献：

1.interleukin 2 receptor: relationship to the interleukin 2-binding site.Eur J Immunol. 17 (7): 929-35.2.Hashimoto, N.et al.(1986) Dissociation of interleukin 2-dependent and -independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody.Eur J Immunol. 16 (3): 317-20.3.Lowenthal, J.W.et al.(1985) High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61.J Immunol. 135 (6): 3988-94.4.Ceredig, R.et al. (1985) Expression of interleukin-2 receptor as a differentiation marker on intrathymic stem cells.Nature 314: 98-100.5.Yaqoob, P. & Calder, P.C. (1997) Glutamine requirement of proliferating T lymphocytes.Nutrition. 13 (7-8): 646-51.6.Scotland, R.S.et al.(2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.Blood. 118 (22): 5918-27.7.Karali, D.et al.(2016) T cell regulation byPhlomis lanataprotein extracts in mice.Pharm Biol. 54 (2): 207-14.8.Szuster-Ciesielska, A.et al.(2019) Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2ofPlasmodium falciparumin a Mouse Model.J Immunol Res. 2019: 9264217.9.Curina, G.et al.(2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuatedBrucella melitensis.REV1 vaccine produced in bioreactor.Vet Immunol Immunopathol. 198: 44-53.10.Arad, T.et al.(2021) CD200 -dependent and -independent immune-modulatory functions of neural stem cells.Stem Cell Res. 56: 102559.

技术参数

产品应用 Flow

产品类型 单克隆抗体

推荐用于 Flow

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