| 产品介绍 | | |
| Mouse anti Human CD13 antibody, clone WM15recognizes human CD13 also known as aminopeptidase N. CD13 is a single pass type II glycosylated integral membrane protein with a predicted molecular mass of ~110 kDa and an apparent molecular mass of ~150 kDa expressed by granulocytes, monocytes, fibroblasts, endothelial cells and by myeloid leukaemia cells (Bradstocket al.1985). CD13 acts as a major cell surface receptor for group 1 coronoviruses (Breslinet al.2003) which bind to a critical sequence encompassing amino acid residies 288-295 (Kolbet al.1997).CD13 functions as anaminopeptidaseenzyme, a metalloprotease present as both a membrane bound form and also a soluble aminopeptidase N.Mouse anti Human CD13, clone WM15 inhibits infection of cells by human coronavirus (Lachanceet al.1998) but not hepatitis C virus (Koutsoudakiset al.2006) and inhibits aminopeptidase N activity of the CD13 molecule (Asmunet al.1992). |
| 产品详情 | | |
Target Species | Human |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rhesus Monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Alexa Fluor® 647 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Immunogen | Human AML cells. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
Alexa Fluor®647 | 650 | 665 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| Acknowledgements | The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells or 100ul whole blood |
| 数据 |
| Figure A. FITC conjugated mouse anti human CD11b (MCA551F) and Alexa647® conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti human CD11b (MCA551F) and Alexa647® conjugated mouse anti human CD13 (MCA1270A647). All experiments performed on red cell lysed Human peripheral blood gated on all mononuclear cells in the presence of Human SeroBlock (BUF070A). |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Test |
| Human Seroblock | BUF070B | F | 200 Test |
| 文献 |
| 1. Stolzing, A.et al.(2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies.Mech Ageing Dev. 129: 163-73.2. Silk, K.M.et al.(2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012:172420.3. Negussie, A.H.et al.(2010) Synthesis and in vitro evaluation of cyclic NGR peptide targeted thermally sensitive liposome.J Control Release. 143: 265-73.4. Lassnig, C.et al.(2005) Development of a transgenic mouse model susceptible to human coronavirus 229E.Proc Natl Acad Sci U S A. 102 (23): 8275-80.5. Thielitz, A.et al.(2004) Identification of extra- and intracellular alanyl aminopeptidases as new targets to modulate keratinocyte growth and differentiation.Biochem Biophys Res Commun. 321 (4): 795-801.6. McCormack, E.et al.(2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.Blood. 121 (7): e34-42.7. Fiddler, C.A.et al.(2016) The Aminopeptidase CD13 Induces Homotypic Aggregation in Neutrophils and Impairs Collagen Invasion.PLoS One. 11 (7): e0160108.8. Chaturvedi, C.P.et al.(2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.Stem Cells Int. 2018: 6901761. |
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