产品介绍 类型:单抗同型对照：Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450浓度： 5 µL/Test用法：5 µL (0.25 µg)/test(Flow)产品详细信息Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: Mouse IgG1 K Isotype Control eFluor® 450 has been reported for use in flow cytometric analysis.Applications Tested: This Mouse IgG1 K Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.<br>Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.数据 CD24 Antibody (48-0247-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD24 eFluor® 450 (purple histogram). Cells in the granulocyte gate were used for analysis.推荐产品二抗荧光二抗免疫组化一抗标签抗体参考文献1. TheranosticsGlutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.2. BMC biologyThe Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states.3. OncogeneTFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis.4. Molecular cancerHOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.5. eLifeTGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24- cancer cells.

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