产品介绍 类型:单抗同型对照：Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450浓度： 5 µL/Test用法：5 µL (0.125 µg)/test(Flow)产品详细信息Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.Applications Reported: This 5C3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 5C3 antibody has been pre-titrated and tested by flow cytometric analyis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.数据

CD40 Antibody (48-0409-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD40 eFluor® 450 (purple histogram). Cells in the lymphocyte gate were used for analysis.推荐产品二抗荧光二抗免疫组化一抗标签抗体参考文献1. Stem cell research & therapyComprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.2. OncoimmunologyA novel subset of B7-H3+CD14+HLA-DR-/lowmyeloid-derived suppressor cells are associated with progression of human NSCLC.3. OncotargetSurrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.4. PloS oneInnate Response Activator (IRA) B Cells Reside in Human Tonsils and Internalize Bacteria In Vitro.5. OncoimmunologyA novel subset of B7-H3+CD14+HLA-DR-/lowmyeloid-derived suppressor cells are associated with progression of human NSCLC.

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