CHT ceramic hydroxyapatite (Ca5(PO4)3OH)2 support is a leading purification media for today’s demanding downstream process industry. Type I CHT media provides high protein binding capacity.
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CHT Ceramic Hydroxyapatite Type I Media | Bio-Rad Laboratories
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CHT Ceramic Hydroxyapatite (Ca5(PO4)3OH)2 support is a leading purification medium for today’s demanding downstream process industry. This mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT media for host cell proteins, leached protein A, antibody dimers and aggregates, nucleic acids, and viruses allow its use at any stage from initial capture to final polishing.
The robust properties of CHT Ceramic Hydroxyapatite improve efficiency, yield, and financial value through:
Excellent capture at elevated flow rates, enabling processing at all scales
Large capacity for high-titer upstream feedstocks
Exceptional selectivity, allowing a two-step chromatographic process
Two types of CHT Ceramic Hydroxyapatite, Type I and Type II, are available in three particle sizes, 20, 40, and 80 µm. Both types have elution characteristics similar to crystalline hydroxyapatite but also have some important differences:
Type I, sintered at 400°C, has a higher protein binding capacity and better capacity for acidic proteins
Type II, sintered at 700°C, has a lower protein binding capacity but offers better resolution of nucleic acids and certain proteins; it also has a very low affinity for albumin and is particularly well-suited for the purification of many species and classes of immunoglobulins
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