DKK1 Human ELISA Kit ELISA试剂盒,DKK1 Human ELISA Kit/人DKK1 ELISA试剂盒

2024-10-23

DKK1 Human ELISA Kit/人DKK1 ELISA试剂盒

货号:EHDKK1,EHDKK1X5,EHDKK1X10

规格:96 Tests,5X96 Tests,10X96 tests

价格:7238,29891,52892

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

100 pg/mL

检测范围122.9-30,000 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 100 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 Tests

组份

- 预包被96孔板

- 标准品

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameDKK1
Protein aliasesdickkopf 1 homolog, dickkopf related protein-1, Dickkopf-1, dickkopf-1 like, Dkk-1, hDkk-1, SK, dickkopf-like protein 1, Dickkopf-related protein 1

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesDKK-1, DKK1, SK, UNQ492/PRO1008
Gene ID(Human) 22943
UniProt ID(Human) O94907
Gene symbolDKK1

The Human DKK1 ELISA quantitates Hu DKK1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu DKK1.人DKK1 ELISA可以定量人血清,血浆或细胞培养基中的Hu DKK1。 该检测试剂盒将专门识别天然和重组人DKK1。Principle of the method 方法原理The Human DKK1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人DKK1固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.此基因编码的蛋白质是dickkopf家族的成员。 它是一种分泌蛋白,具有两个富含半胱氨酸的区域,并通过抑制WNT信号传导途径参与胚胎发育。 多发性骨髓瘤患者中,血浆和外周血中DKK1水平升高与溶骨性病变有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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