ScienCellGK098人类心脏肌细胞生物学qPCR检测的性能

2024-08-08

GK098 ScienCell 人类心脏肌细胞生物学qPCR检测试剂盒,Human Cardiac Myocyte Biology qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京作为ScienCell品牌的特约代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK098 ScienCell人类心脏肌细胞生物学qPCR检测试剂盒,Human Cardiac Myocyte Biology qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK098

产品名称:GK098 ScienCell人类心脏肌细胞生物学qPCR检测试剂盒,Human Cardiac Myocyte Biology qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

北京裕恒丰科技有限公司是ScienCell中国区总代理,提供各种基因表达分析相关品,ScienCell细胞培养基、转染试剂、细胞检测试剂盒等细胞生物学产品。

GK098 ScienCell人类心脏肌细胞生物学qPCR检测试剂盒,Human Cardiac Myocyte Biology qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ 人心肌细胞生物学 qPCR 阵列试剂盒 (GQH-CMB) 旨在促进参与人类心肌细胞生物学的 88 个关键基因的基因表达谱分析,例如心肌收缩、心肌细胞增殖和分化以及心脏 z 盘信号网络。与选定的心肌细胞相关疾病有关的基因也包含在阵列中。下面显示了如何根据其功能对基因进行分组的简要示例:

心肌细胞标志物:ACTN1、TNNT2、NKX2-5、GJA1、NPPA、MYH6、MYH7B、TNNI3、CAV3、GATA4、MYL2、MEF2C

心肌收缩: CAMK2s, ATP2As, MYH7, MYL2, CACNA1C, HRC, ITPR3, KCNJ5, TRDN, TPM

心脏Z盘信号网络:AGTR1,ACTN,CSRP3,MYOZ2,ITGB1BP2,DES,ITGA1,ITGB1,ILK,CACNA1s,CIB1,MYOZ2/3

心肌细胞发育和增殖:GATA4,HAND2,MEF2C,TBX5,FOXP1,NKX2-5,FGFs,PIK3CB,SAV1,STK3 / 4,YAP1,WWTR1

心肌细胞分化:肌细胞分化,TWIST1,VANGL2,BCKDHA,CPT1B,GPAM,POU5F1,MEF2C,NPPA/B,MYOG

与以下因素有关的基因

肥厚型心肌病: MYH6/7, MYBPC3, TNNI3, PMPCA, TNNT2, TPM1, ACTC1, MYL2/3

心肌梗死: OLR1, LRP8, LTA, LGALS2, ESR1, FABP6

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Cardiac Myocyte Biology qPCR Array Kit (GQH-CMB) is designed to facilitate gene expression profiling of 88 key genes involved in human cardiac myocyte biology, such as cardiac muscle contraction, cardiac myocyte proliferation and differentiation, and cardiac z-disk signaling network. Genes implicated in selected cardiac myocyte related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:

· Cardiac myocyte markers: ACTN1, TNNT2, NKX2-5, GJA1, NPPA, MYH6, MYH7B, TNNI3, CAV3, GATA4, MYL2, MEF2C

· Cardiac muscle contraction: CAMK2s, ATP2As, MYH7, MYL2, CACNA1C, HRC, ITPR3, KCNJ5, TRDN, TPMs

· Cardiac z-disk signaling network: AGTR1, ACTNs, CSRP3, MYOZ2, ITGB1BP2, DES, ITGA1, ITGB1, ILK, CACNA1s, CIB1, MYOZ2/3

· Cardiac myocyte development and proliferation: GATA4, HAND2, MEF2C, TBX5, FOXP1, NKX2-5, FGFs, PIK3CB, SAV1, STK3/4, YAP1, WWTR1

· Cardiac myocyte differentiation: MYOCD, TWIST1, VANGL2, BCKDHA, CPT1B, GPAM, POU5F1, MEF2C, NPPA/B, MYOG

· Genes implicated in

o hypertrophic cardiomyopathy: MYH6/7, MYBPC3, TNNI3, PMPCA, TNNT2, TPM1, ACTC1, MYL2/3

o myocardial infarction: OLR1, LRP8, LTA, LGALS2, ESR1, FABP6

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人心肌细胞生物学 qPCR 阵列板布局*(8 个粗体和斜体控件)

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTN1

ATP2A3

CAMK2D

FABP6

GLRX3

ITPR3

MYBPC3

MYOZ2

PMPCA

SLC9A1

TPM2

β-actin

B

ACTN2

BCKDHA

CAMK2G

FGF10

GPAM

KAT2B

MYH6

MYOZ3

POU5F1

STK3

TPM3

GAPDH

C

ACTC1

CACNA1C

CAV3

FGF16

HAND2

KCNJ5

MYH7

NKX2-5

PRKCB

STK4

TPM4

LDHA

D

ACTN3

CACNA1D

CIB1

FGF20

HRC

LGALS2

MYH7B

NPPA

QDPR

SYNPO2

TRDN

NONO

E

ACTN4

CACNA1F

CPT1B

FGF3

ILK

LMCD1

MYL2

NPPB

RYR2

TBX5

TWIST1

PPIH

F

AGTR1

CACNA1S

CSRP3

FOXP1

ITGA1

LRP8

MYL3

OLR1

SAV1

TNNI3

VANGL2

GDC

G

ATP2A1

CAMK2A

DES

GATA4

ITGB1

LTA

MYOCD

PAK1

SCN5A

TNNT2

WWTR1

PPC

H

ATP2A2

CAMK2B

ESR1

GJA1

ITGB1BP2

MEF2C

MYOG

PIK3CB

SLC8A1

TPM1

YAP1

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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GK029 Human Tie2/Angpt信号qPCR检测试剂盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034 人肺纤维化qPCR检测试剂盒,Human Pulmonary Fibrosis qPCR Array Kit

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