ScienCellGK090人体细胞连接qPCR检测试剂盒

2024-08-08

GK090 ScienCell 人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK090 ScienCell人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK090

产品名称:GK090 ScienCell人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK090 ScienCell人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ 人类细胞连接 qPCR 阵列试剂盒 (GQH-JXN) 分析了参与细胞连接形成、组织和维护的 88 个关键基因。 该试剂盒可研究对细胞通讯、细胞旁转运和锚定很重要的细胞-细胞连接。 如何对所包含的基因进行分类的简要示例如下所示:

间隙连接点: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1

粘附连接: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2

紧密结: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)

桥粒: DSC1, DSG1, DSP, ITGB4, JUP

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Cell Junctions qPCR Array Kit (GQH-JXN) profiles 88 key genes involved in cell junction formation, organization, and maintenance. This kit surveys cell-cell junctions that are important for cellular communication, paracellular transport, and anchoring. Brief examples of how included genes may be categorized are shown below:

· Gap junctions: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1

· Adherens junctions: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2

· Tight junctions: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)

· Desmosomes: DSC1, DSG1, DSP, ITGB4, JUP

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

基因查询™人类细胞连接 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

CADM1

CDH19

CELSR3

CLDN8

CXADR

DSP

GJA5

JAM3

NECTIN3

PECAM1

β-actin

B

ACTA2

CADM3

CDH2

CGN

CLTA

DCHS1

F11R

GJB1

JUP

OCLN

PVR

GAPDH

C

ACTB

CDH1

CDH3

CLDN1

CLTB

DCHS2

FAT1

GJB6

LEF1

PAK1

RHOA

LDHA

D

ACTC1

CDH10

CDH5

CLDN10

CLTC

DNM1

FAT2

ICAM1

MAGI1

PANX1

SNAI1

NONO

E

ACTG1

CDH11

CDX1

CLDN2

CLTCL1

DSC1

FAT3

ILK

MAGI2

PARD3

SP1

PPIH

F

ACTG2

CDH13

CDX2

CLDN4

CSNK1D

DSC2

FAT4

ITGB1

MYLK

PARD6A

SRC

GDC

G

ANG

CDH15

CELSR1

CLDN5

CTNNB1

DSG1

GJA1

ITGB4

NECTIN1

PCDH7

TJP1

PPC

H

AP2M1

CDH16

CELSR2

CLDN7

CTNND1

DSG3

GJA4

JAM2

NECTIN2

PCDH8

WNK4

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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