GK091 ScienCell 人肝星状细胞生物学qPCR检测试剂盒，Human Hepatic Stellate Cell Biology qPCR Array Kit

ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。

北京作为ScienCell品牌的代理商，为客户提供，正规进口，保证原装正品，货期稳定，折扣好的服务标准。

GK091 ScienCell人肝星状细胞生物学qPCR检测试剂盒，Human Hepatic Stellate Cell Biology qPCR Array Kit，产品简介：

产品品牌： ScienCell

产品货号：GK091

产品名称：GK091 ScienCell人肝星状细胞生物学qPCR检测试剂盒，Human Hepatic Stellate Cell Biology qPCR Array Kit

产品规格：One 96-well plate, one 20ul reaction per well

GK091 ScienCell人肝星状细胞生物学qPCR检测试剂盒，Human Hepatic Stellate Cell Biology qPCR Array Kit， 产品说明：

ScienCell 的 GeneQuery™ 人肝星状细胞生物学 qPCR 芯片试剂盒 （GQH-HSB） 旨在促进 88 个关键基因的基因表达谱分析，这些基因涉及 （i） 人肝星状细胞活化和增殖;（二）视黄醇代谢;（iii）细胞外基质（ECM）合成和重塑;以及（iv）肝星状细胞相关疾病。如何根据其功能对所包含的基因进行分组的简要示例如下所示：

肝星状细胞标志物：ACTA2，GFAP，DES，NGFR，COL2A1，VIM，LAMA1，NES

肝星状细胞活化和增殖：ICAM1，MMP13，RELN，TIMP1，SYP，CDH1，RARA，PDGF，TGFB1，MMP2

肝脏发育和再生：STX2，PTN，SYNM，FOXF1，ICAM1，VCAM1，ITGB3，HGF，CXCL12，DES

视黄醇代谢： RBP1， RBP2， RBP4， RBP5， CRABP1， CRABP2， LRAT， CYP26

ECM合成和重塑：TGFB1，COL4A2，层粘连蛋白亚基，SPARC，VCAN，CTGF

细胞因子生产：EGF，IGF，PDGF，FGF，CSF1，CCL2，TNF，CXCL2，CXCL3，CXCL8

肝纤维化和癌症发展：PKHD1，TMEM67，RPGRIP1L，SLC17A5，PKD1，CC2D2A，B9D1，B9D2

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪，每个孔包含一个引物组，可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保：（i）qPCR分析中的最佳退火温度为65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有说明，否则引物组可识别靶基因的所有已知转录本变体;（iii）只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:

· Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES

· Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2

· Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES

· Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26

· ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF

· Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8

· Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人肝星状细胞生物学qPCR阵列板布局*（8 个粗体和斜体控件）

提示：基因选择可能会根据新的研究和开发进行更新

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